Fig. 8 | Nature Communications

Fig. 8

From: Galectin-3 captures interferon-gamma in the tumor matrix reducing chemokine gradient production and T-cell tumor infiltration

Fig. 8

Galectin antagonists delay tumor growth. a Scheme showing the protocol for the analysis of tumor growth. s.c. stands for subcutaneous injection, I.T. for intratumoral injection, I.V. for intravenous injection, and I.P. for intraperitoneal injection. The doses used for the I.T. treatment were IFNγ 50 ng per tumor, LacNAc 0.1 μmol per tumor, and antibodies 100 ng per tumor. The doses used for the I.P. boosts were IL-2 200 ng per mouse, LacNAc 40 μmol per mouse, and antibodies 50 μg per mouse. b Tumor growth in mice untreated or treated with either IFNγ and a control isotype antibody or IFNγ  + LacNAc + αGal-3 antibody (n = 7, n = 10, and n = 11, respectively; mean ± SEM). Two-way ANOVA ****P < 0.0001, where treatment has F = 19.83, and degree of freedom = 1, and time has F = 24.91, and degree of freedom = 7. Individual tumor growths are shown in Supplementary Fig. 12. c Tumor growth in mice treated with IFNγ and a control isotype antibody, IFNγ and αGal-3 antibody or only with the αGal-3 antibody (n = 17, n = 18, and n = 10 respectively; mean ± SEM). Two-way ANOVA ****P < 0.0001, where treatment has F = 40.26, and degree of freedom = 1, and time has F = 13.52, and degree of freedom = 7. Individual tumor growths are shown in Supplementary Fig. 12