Fig. 1 | Nature Communications

Fig. 1

From: Galectin-3 captures interferon-gamma in the tumor matrix reducing chemokine gradient production and T-cell tumor infiltration

Fig. 1

Binding of human glycosylated cytokines to galectin-3-coated beads. Ratio refers to molar ratio. a hIFNγ measured by ELISA in the supernatant after incubation with galectin-3-coated beads in the presence or absence of 100 mM lactose. The glycosylated IFNγ was produced in CHO cells and the unglycosylated IFNγ was produced in E. coli. Mean ± SD of one representative experiment of 8 (glycosylated IFNγ) or 2 (unglycosylated IFNγ), performed in duplicates. The dotted line stresses that more than 90% of the glycosylated IFNγ was retained when mixed with 10 times more galectin-3-coated beads. b Glycosylated IFNγ measured by ELISA in the supernatant after incubation with galectin-3-coated beads and different galectin antagonists (LacNAc 5 mM, TetraLacNAc 30 μM, GM-CT-01 100 μg ml−1), anti-galectin-3 antibody or a control isotype (10 μg ml−1). Mean ± SD of one representative experiment of three. c Glycosylated hIL-12 measured by ELISA in the supernatant after incubation with galectin-3-coated beads in the presence or absence of 100 mM lactose. Mean ± SD of one representative experiment of three, performed in duplicates. d Cytokines and chemokines measured in a human synovial fluid collected from a rheumatoid arthritis patient after incubation with galectin-3-coated beads in the presence or absence of 100 mM lactose. Note: glycosylated cytokines and chemokines were entirely glycosylated, containing no detectable unglycosylated fraction

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