Fig. 1 | Nature Communications

Fig. 1

From: Measuring the effects of particle orientation to improve the efficiency of electron cryomicroscopy

Fig. 1

Particle orientation distributions can be described by their efficiency, E od. A uniformly distributed set of 10,000 particle orientations a provides uniform coverage of Fourier space d, which corresponds to a spherically symmetric PSF with a narrow distribution of radii g and high E od. A distribution with 1/3 of the views missing b, corresponds to a cone of missing density in Fourier space e, an elongated PSF with a larger variation in radii h and a reduced E od. A third distribution with 2/3 of the views missing (equiv. to a strongly preferred orientation) c corresponds to a large empty cone in Fourier space f, a strongly elongated PSF with a bimodal distribution of radii i and a low E od. The efficiency is linearly related to the fraction of Fourier space covered by the orientation distribution (j, inset). The root mean square error (RMSD) in efficiency is plotted vs. number particles used for the estimation j. The simulated relationship between the mean resolution and the number of particles used for a reconstruction is plotted k for different orientation distributions and B-factors

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