Fig. 3 | Nature Communications

Fig. 3

From: A single-dose live-attenuated vaccine prevents Zika virus pregnancy transmission and testis damage

Fig. 3

ZIKV-3′UTR-∆10-LAV and ZIKV-3′UTR-∆20-LAV protect rhesus macaques (RMs) from ZIKV infection. a Scheme of immunization of RMs with 103 FFU of WT ZIKV strain FSS13025 (n = 4), ZIKV-3′UTR-∆10-LAV (∆10; n = 4), ZIKV-3′UTR-∆20-LAV (∆20; n = 3), ZIKV-NS1-LAV or PBS sham (n = 2) via the subcutaneous route. b Viremia was measured at days 2, 3, 4, 5, 7, and 10 post immunization by qRT-PCR. Each colored line represents data from a different animal in each group. The dashed line indicates the limit of detection (LOD) of the assay. c Pre- and post-challenge antibody neutralization titers. On various days post immunization, sera were measured for neutralizing titers using an mCherry ZIKV infection assay. Red arrows indicate challenge with 103 FFU of epidemic ZIKV strain PRVABC59 via the subcutaneous route at day 56 post immunization. The number of animals whose antibody neutralization titers increased by ≥ 4-fold after challenge is indicated by symbol “↑” for each experimental group. d Post-challenge viremia. Viremia was measured by qRT-PCR at days 2, 3, 4, 5, 7, and 10 post challenge. All negative samples are plotted at the half value of LOD. Error bars represent SDs

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