Fig. 9 | Nature Communications

Fig. 9

From: RSV glycoprotein and genomic RNA dynamics reveal filament assembly prior to the plasma membrane

Fig. 9

RSV genomic RNA is assembled with extended filaments. a Vero cells were infected with RSV at an MOI of 1. At 12 hpi, Cy3B-labeled MTRIPs (red) targeted to RSV genomic RNA were delivered at 30 nM. After membrane recovery, SBA-488 (green) was delivered and allowed to internalize for 1 h. Cells were then imaged live at 4.3 Hz. Images were smoothed with a fine rolling ball filter in Volocity. Scale bar represents 1 µm. b Vero cells were infected with RSV at an MOI of 1. At 12 hpi, Cy3B-labeled MTRIPs (red) targeted to RSV genomic RNA were delivered at 30 nM. After membrane recovery, SBA-488 (green) was delivered and allowed to internalize for 1 h. Microtubules were then disrupted using nocodazole. Cells were then imaged live at 4.2 Hz. Scale bar represents 1 µm