Fig. 6 | Nature Communications

Fig. 6

From: RSV glycoprotein and genomic RNA dynamics reveal filament assembly prior to the plasma membrane

Fig. 6

RSV filaments are formed by a rapid vesicular extension and retraction motion. a Vero cells were infected with RSV A2 at an MOI of 1. At 16 hpi, SBA-488 was delivered and allowed to internalize for an hour. Cells were then imaged live at 12.13 Hz. Time series are shown with either even temporal spacing (top) or as selected frames from the movie (bottom). Yellow arrowheads indicate distension of vesicles, while arrows indicate extended filaments and branched filaments. Scale bar represents 5 µm. b Individual extension and retraction events were analyzed by hand on a frame-by-frame basis to determine the mean velocity. 30 events of each type were analyzed. Error bars represent standard deviation. c Vero cells were infected with RSV A2 at an MOI of 1. After 12 h, SBA-488 (green) was delivered to the cells and allowed to internalize for 1 h before being imaged every 10 min. Cropped regions of two individual filaments are shown. Yellow arrows mark the two apparent ends of the filaments. Scale bars represent 1 µm