Fig. 5 | Nature Communications

Fig. 5

From: RSV glycoprotein and genomic RNA dynamics reveal filament assembly prior to the plasma membrane

Fig. 5

RSV G protein internalizes via clathrin and colocalizes with caveolin in viral filaments. a RSV infected Vero cells were stained for RSV G with SBA-488 (green). The cells were incubated at 37 °C before being fixed and stained for CHC at the indicated time points (red). The cell nuclei were stained with DAPI (blue). Scale bar represents 10 µm. b Enlargements of cropped images from the white boxed regions in part a with intensity profiles showing colocalization along the direction of the white arrow. c Mander’s colocalization coefficient and Pearson’s correlation between RSV G and CHC. Dotted line indicates 0 correlation. Error bars represent standard deviation. Asterisk indicates p < 0.05 (Mann–Whitney U test). n = 30 cells per group. d Infected Vero cells at 12 hpi were treated with the indicated concentration of chlorpromazine in media for 2 h. RSV G was labeled with SBA-488 at 4 °C followed by a 1 h incubation at 37 °C in media with drug. Cells were fixed and stained for CHC. Scale bar represents 10 µm. Enlargements of cropped images from the white boxed regions with intensity profiles showing colocalization along the direction of the white arrow. e Mander’s colocalization coefficient between RSV G and CHC. Error bars represent standard deviation. Asterisk indicates p < 0.05 (Mann–Whitney U test). n = 30 cells per group. f Vero cells were infected with RSV A2 at on MOI of 1. At 24 hpi, RSV G was labeled with SBA-488 (green) and cells were fixed and stained for caveolin (red) and nuclei (blue). Enlargement of cropped image from the white boxed region with an intensity profile showing colocalization along the direction of the white arrow. Scale bar represents 10 µm