Fig. 4 | Nature Communications

Fig. 4

From: RSV glycoprotein and genomic RNA dynamics reveal filament assembly prior to the plasma membrane

Fig. 4

RSV G recycles from the plasma membrane into intracellular vesicles. a Vero cells were infected with RSV A2 at an MOI of 1. Cells were either labeled with SBA-488 (green) at 12, 18, and 24 hpi (top) or with SBA-647 (red) at 12 hpi and SBA-488 at 24 hpi. Cells were then fixed and stained for RSV F (blue). Enlargements of cropped images of either filaments or intracellular vesicles from the white boxed regions with intensity profiles showing colocalization along the direction of the white arrow. Scale bar represents 10 µm. b Vero cells were infected with RSV for 12 hpi before being labeled with SBA-488 (green). Immediately following SBA delivery, cells were imaged live over 1 h. Extended focus images shown. Scale bars represent 10 µm

Back to article page