Fig. 1 | Nature Communications

Fig. 1

From: RSV glycoprotein and genomic RNA dynamics reveal filament assembly prior to the plasma membrane

Fig. 1

SBA selectively binds RSV G. a Vero cells infected (top) or mock-infected (bottom) for 24 h with RSV A2 at an MOI of 1 were labeled with SBA-488 at 4 °C (green) before being fixed and stained for RSV G (red), RSV F (blue), and nuclei (gray). Extended focus image is shown. Scale bar represents 10 µm. b Enlarged cropped images from the white boxed regions from part a with intensity profiles showing colocalization along the direction of the white arrow. Scale bar represents 1 µm. c Cells transfected for 24 h with RSV G (top) and RSV F (bottom) mRNA were labeled with SBA-488 at 4 °C (green) before being fixed and stained without permeabilization for RSV G or RSV F (red) and nuclei (gray). Extended focus image is shown. Scale bar represents 15 µm. d Mander’s colocalization coefficient and Pearson’s correlation between RSV G or RSV F and SBA. Dotted line indicates 0 correlation. n = 16 cells per group. Error bars represent 95% confidence interval. Asterisks indicates p < 0.0001 by Mann–Whitney U Test. e Enlarged cropped images from the white boxed regions from part c with YZ cross-sections (left). Intensity profiles, indicated by the white line on the YZ cross-section, show the expressed RSV protein on a higher plane than the nucleus as indicated by the arrow. Extended focus cropped images are shown