Fig. 6 | Nature Communications

Fig. 6

From: RNA localization is a key determinant of neurite-enriched proteome

Fig. 6

A subset of RBPs localizes to neurites. a Neurite-localized RBPs. Proteomic data from Fig. 1e were overlaid with available databases of mRNA-bound proteins48,49,50,51. Neurite-localized RBPs are highlighted in green (protein neurite/soma log2FC > 1, P-values < 0.05; see also Supplementary Data 2), the rest of RBPs are shown in blue. b Western blot validation for selected neurite-enriched RBPs. Histone H3 and TUBB3 were used as soma-enriched markers. c Manual annotation of neurite-enriched RBPs (protein neurite/soma log2FC > 1 and P-values < 0.05) for RNA-related functions. Number of proteins in a given category is indicated on the pie chart. Some RBPs were annotated to multiple GO categories. See also Supplementary Data 7. d Motifs found in mRNAs localized to neurites and locally translated. Motif discovery was done with MEME56 and enrichment calculations with MAST57. Fisher’s exact test was used to assess statistical significance of the association and its enrichment (odds ratio). Alignment of known RBP target sites70 (not restricted to neurite-localized RBPs identified in Fig. 6a) was performed using Tomtom60; only best hits are shown

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