Fig. 2 | Nature Communications

Fig. 2

From: RNA localization is a key determinant of neurite-enriched proteome

Fig. 2

RNA localization determines protein localization to neurites. a Correlation heatmap of individual RNA-seq libraries prepared from neurites and soma of iNeurons (three biological replicates). Numbers indicate Pearson’s correlation coefficients. b Local transcriptome from neurites and soma. RNA-seq data are presented as RPKM (reads per kilobase of transcript per million mapped reads) and shown as in Fig. 1d. c qRT-PCR for selected neurite-localized RNAs identified by RNA-seq. Histone RNAs and rRNA used as soma-localized and unlocalized controls. Error bars represent SD. d RNA and protein enrichement in neurites of iNeurons. Green: neurite-localized proteins and RNAs (log2FC > 1, P-values < 0.05); blue: soma-localized proteins and RNAs (log2FC < −1, P-values < 0.05). e Average protein enrichment in neurites plotted for the group of genes localized at both protein and RNA level (red) and genes that are localized only at the protein level (blue; log2FC > 1, P-values < 0.05)