Fig. 1 | Nature Communications

Fig. 1

From: RNA localization is a key determinant of neurite-enriched proteome

Fig. 1

Local proteome of iNeurons. a Separation scheme. iNeurons are grown on a microporous membrane so that cell outgrowths extend on the lower coated side of the membrane to enable separation of the two cellular compartments (soma and neurites). b Fluorescent micrographs of the iNeurons differentiated on a microporous membrane described in a. Images taken above (top) and below (bottom) the membrane. Neurofilament immunostained neurites (green) extend on the lower side of the membrane, whereas soma (DAPI, blue) remain on the top, scale bar = 50 μm. The insert shows the magnification of the membrane with neurites growing through the membrane pores (green), scale bar = 5 μm. c Correlation heatmap of mass spectrometry replicates prepared from neurites and soma of iNeurons (three biological replicates in each case). Mass spectrometry samples were quantified using a label-free quantification method (LFQ). The numbers represent Pearson correlation coefficients of LFQ values. d, e Local proteome from neurites and soma. The data are presented as protein enrichment in neurites versus soma plotted against average LFQ intensities (left) and as a volcano plot (right). Green: neurite-localized proteins (log2FC > 1, P-values < 0.05); blue: soma-localized proteins (log2FC < −1, P-values < 0.05)

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