Fig. 7 | Nature Communications

Fig. 7

From: A network integration approach for drug-target interaction prediction and computational drug repositioning from heterogeneous information

Fig. 7

The real-time PCR (RT-PCR) analyses of the proinflammatory factors on the LPS-stimulated macrophages. af The RT-PCR analysis of mRNA expressions of TNFα, IL-6, IL-1β, IL-12p35, CXCL-1 and iNOS normalized relative to that of GAPDH, respectively. Control, macrophages without LPS treatment. *: P < 0.05; **: P < 0.01; ***:P < 0.001, compared to the samples without LPS treatment. ##: P < 0.01; ###:P < 0.001, compared to the samples treated with LPS. n = 3. Newman–Keuls multiple comparison test was used. Here, data show the mean with the standard deviation of three independent experiments, each of which was performed with triplicates. The concentrations of the COX inhibitors were determined according to the indications of the assay kits and the previous binding studies in the literature (see Methods)

Back to article page