Table 1: Kinetic constants for macromolecular or peptidyl substrate cleavage and prothrombinase assembly

From: Engineered factor Xa variants retain procoagulant activity independent of direct factor Xa inhibitors

  Prothrombina Prothrombina Cofactor Vaa S2765a S2765a S2765b S2765b
  K m (µM) k cat (min−1) K d, app (nM) K m (µM) k cat (s−1) K m (µM) k cat (s−1)
pd-FXa 0.31 ± 0.07 1880 ± 136 0.41 ± 0.05 59 ± 19 47 ± 4  29 ± 8 36 ± 3
wt-FXa 0.41 ± 0.08 1243 ± 89 1.44 ± 0.41 33 ± 7 27 ± 2  26 ± 8  21 ± 2
FXa-A 0.14 ± 0.04  122 ± 8 0.81 ± 0.26 39 ± 7  9 ± 1 243 ± 50   11 ± 1
FXa-B 0.25 ± 0.07  239 ± 20 0.85 ± 0.28 49 ± 8 10 ± 1 249 ± 29  15 ± 1
FXa-C 0.22 ± 0.04  370 ± 19 0.57 ± 0.06 60 ± 11 17 ± 1  216 ± 28  21 ± 1