Figure 2: Nature Communications

Fig. 2

From: Engineered factor Xa variants retain procoagulant activity independent of direct factor Xa inhibitors

Fig. 2

Functional characterization of the effect of the direct factor Xa inhibitors on factor Xa paralogs. a The rate of peptidyl substrate conversion by purified recombinant P. textilis venom FXa (10 nM; squares) or plasma-derived human FXa (2 nM; circles) was determined in the absence (V 0) or presence (V i) of increasing concentrations (0.01–100 µM) of apixaban (APX; open symbols) or rivaroxaban (RVX; closed symbols). b The rate of peptidyl substrate conversion by RVV-X activated P. textilis venom FX (closed squares), isoform FX (semi-closed diamonds), or liver FX (open diamonds) was determined in the absence (V 0) or presence (V i) of increasing apixaban concentrations (0.01–100 µM). a, b The lines were drawn following nonlinear regression analysis of the data sets, and the fitted parameters for IC50 are shown in the inset. The data are the means of two independent experiments. *For these experiments, FXa inhibition was inefficient, precluding an accurate assessment of the IC50 values