Fig. 7 | Nature Communications

Fig. 7

From: Tanycytes control the hormonal output of the hypothalamic-pituitary-thyroid axis

Fig. 7

The tanycyte-specific deficiency of q/11 enhances the TSH release in response to activation of TRH neurons. a Schematic representation of the experimental groups. PVNCon- q/11 Con: Gα q fl/fl::Gα 11 +/− mice were injected with AAV-Dio2-GFP into the LV ( q/11 Con) without injection of an AAV into the PVN (PVNCon; gray). PVN3D- q/11 Con: the PVN of q/11 Con mice were bilaterally injected with AAV-TRH-hM3D-mCherry (PVN3D, red). PVN3D- q/11 tanKO: a deficiency of the q/11 pathway in tanycytes was induced by injecting AAV-Dio2-iCre-2A-GFP in the LV of q fl/fl:: 11 −/− mice. In parallel, AAV-TRH-hM3D-mCherry was bilaterally injected into the PVN to generate PVN3D- q/11 tanKO mice (purple). All mice received CNO. be TRH-dependent parameters 2 h after CNO (0.5 mg kg−1 body weight) administration to PVNCon- q/11 Con, PVN3D- q/11 Con, and PVN3D- q/11 tanKO mice. b TSH plasma levels. One-way ANOVA, F(2/22) = 23.6, p < 0.0001. **p < 0.01, ***p < 0.005 between indicated groups (Bonferroni post test). c T4 plasma levels. One-way ANOVA, F(2/24) = 3.4, p = 0.05. *p < 0.05 between indicated groups (Bonferroni post test). d T3 plasma levels. One-way ANOVA, F(2/23) = 3.7, p = 0.042. p > 0.05 between groups (Bonferroni post test). e Tshb mRNA expression in the pituitary. One-way ANOVA, F(2/23) = 4.3, p = 0.027. *p < 0.05 between indicated groups (Bonferroni post-test). f As a measure of TSH activity, the intracellular cAMP concentration in CHO JP26 cells was determined 1 h after stimulation with plasma of PVNCon- q/11 Con, PVN3D- q/11 Con, or PVN3D- q/11 tanKO mice. Plasma was collected 2 h after CNO stimulation. One-way ANOVA, F(2/22) = 12.7, p = 0.0003. *p < 0.05 between indicated groups (Bonferroni post test). g Linear correlation of TSH plasma concentrations (from b) and TSH activity expressed as stimulated cAMP concentrations (from f). R 2 = 0.5861, p < 0.0001 (Pearson correlation). h Thrsp mRNA expression in the liver. One-way ANOVA, F(2/21) = 4.3, p < 0.0004. *p < 0.05, **p < 0.001 between indicated groups (Bonferroni post test). i Fasn mRNA expression in the liver. Kruskal–Wallis test, 10.76, p < 0.0046. *p < 0.05 between indicated groups (Dunn’s post test). mean ± S.E.M.; n as indicated