Fig. 5 | Nature Communications

Fig. 5

From: Tanycytes control the hormonal output of the hypothalamic-pituitary-thyroid axis

Fig. 5

Activation of the Gαq/11 pathway induces changes in tanycytic endfoot diameters. a To express hM3D-mCherry in tanycytes, we injected AAV-CAG-flex(hM3D-mCherry) plus AAV-Dio2-iCre-2A-GFP in the LV of C57Bl/6 (dTan3D-Bl6) or q fl/fl:: 11 −/− (dTan3D- q/11 tanKO) mice (2 × 1010 genomic particles of each vector in a total volume of 2.5 µl). One hour after treating mice with 0.9% NaCl (dTan3D-Bl6, n = 8; dTan3D- q/11 tanKO, n = 7), CNO (n = 7) or taltirelin (dTan3D-Bl6, n = 8; dTan3D- q/11 tanKO, n = 7), the diameters of 18–26 tanycytic endfeet were quantified. Mean diameters per animal are presented. One-way ANOVA, F(4/32) = 29.47, p < 0.0001; ***p < 0.0001 between indicated groups (Bonferroni post test); mean ± S.E.M. b Representative images of tanycytic endfoot morphology (yellow arrowheads) for all groups (mCherry, gray; collagen IV, red). Scale bar, 2 µm