Fig. 3 | Nature Communications

Fig. 3

From: Tanycytes control the hormonal output of the hypothalamic-pituitary-thyroid axis

Fig. 3

Morphology of tanycyte endfeet is modulated by the q/11 pathway. ae Characterization of the ME structure in coronary sections. Tanycytes were labeled with the membrane-bound protein hM3D-mCherry by injecting AAV-CAG-flex(hM3D-mCherry) in the lateral ventricle of GlastCreER T2 mice and inducing the Cre recombinase with tamoxifen (gTam3D). Beta-tanycytes have projections that are branched in their terminal third. The endings of the branches, the so-called endfeet, terminate on the surface of the fenestrated vessels of the portal venous system, located at the bottom of the ME (a; red, tanycytes, hM3D-mCherry; green, vessels, collagen IV). Scale bar, 25 µm. b High-magnification image of tanycytic endfeet in gTam3D mice. Scale bar, 5 µm. c Astrocytes are present in a layer beneath the tanycytic cell bodies (cyan, astrocytes, aquaporin 4). Scale bar, 50 µm. d Neuronal terminals are located in the central part of the ME (yellow, neuronal terminals, neurofilament 200). Scale bar, 50 µm. e Schematic drawing of the ME structure. f CNO (10 µM, gray bar) increased tanycytic [Ca2+]i in acute brain slices of gTan3D mice (red) in comparison to untransduced control mice (gTanCon, black). Slices were loaded with the calcium responsive dye Fura-2. Representative traces are shown. g Quantitative analysis of tanycytic endfoot diameters in gTan3D mice 1 h after injecting 0.9% saline (gray; NaCl; i.p.) or CNO (red, i.p.). Mean endfoot diameters were determined from 15 to 32 endfeet per animal. *p = 0.0147 (two-tailed Student’s t-test); mean ± S.E.M.; n, number of animals as indicated. h and i Representative images of tanycytic endfeet (arrowheads) in gTan3D mice 1 h after injecting the vehicle (h, 0.9% NaCl) or CNO (i). Scale bar, 2 µm