Fig. 2 | Nature Communications

Fig. 2

From: Tanycytes control the hormonal output of the hypothalamic-pituitary-thyroid axis

Fig. 2

TRHR1 activates the Gαq/11 pathway selectively in tanycytes of the median eminence. a and b Quantification of taltirelin- (33 µM; a) and ATP- (33 µM, b) induced maximal [Ca2+]i response (max F/F0) after treatment with the TRHR antagonist midazolam (500 µM) and in Trhr1 −/− and Trhr2 −/− mice. n, as indicated; *p < 0.05 vs. taltirelin stimulation (Kruskal–Wallis with post hoc Dunn’s test); mean ± S.E.M. c Representative heat maps after stimulation with taltirelin (33 µM, top) and ATP (33 µM, bottom) in acute brain slices of GCamP6s-transduced Trhr1 −/− mice. Scale bar, 50 µm. d Quantification of taltirelin-induced maximal [Ca2+]i response under pharmacological and genetic inhibition of the Gαq/11 pathway ( q/11 gliaKO). n, as indicated; *p < 0.05 vs. taltirelin stimulation (Kruskal–Wallis with post hoc Dunn’s test); mean ± S.E.M. e and f Representative [Ca2+]i responses upon taltirelin stimulation (33 µM, gray bar) before (left curve) and after (right curve) treatment with the PLC blocker U73122 (100 µM; 30 min preincubation; e) or the IP3-receptor blocker 2-APB (100 µM; 30 min preincubation; f)