Fig. 1 | Nature Communications

Fig. 1

From: Tanycytes control the hormonal output of the hypothalamic-pituitary-thyroid axis

Fig. 1

TRH receptor agonists increase [Ca2+]i selectively in tanycytes of the median eminence. a and b TRH and the TRH receptor agonist taltirelin increased [Ca2+]i in tanycytes of the ME 2 weeks after rAAV-mediated transduction of the calcium sensor GCamP6s. a After treating hypothalamic slices with TRH (33 µM, gray bar), only β-tanycytes (red line) responded with an increase in [Ca2+]i but not α-tanycytes (black line). Representative traces are shown. b Heat maps of fluorescence intensity visualized the location of responsive cells after taltirelin stimulation (33 µM). Scale bar, 50 µm. c Single-cell calcium responses after stimulation with taltirelin (150 µM, gray bar, top) and ATP (150 µM, gray bar, bottom) in tanycytes of the ME (red, R1–3) and the ventricular wall (gray, R4–6). The regions of interest that were imaged by confocal microscopy are indicated in d (R1–6). d Representative heat maps after stimulation with taltirelin (150 µM, top) and ATP (150 µM, bottom) in acute brain slices of GCamP6s-transduced mice. Regions of interest are indicated in the ME (R1–3, red) and the ependymal layer of the 3rd ventricle (R4–6; gray). Scale bar, 25 µm