Fig. 5 | Nature Communications

Fig. 5

From: Glycoprotein 2 is a specific cell surface marker of human pancreatic progenitors

Fig. 5

GP2 and CD142 comparative profiles. aj Flow cytometry analyses of H1-derived day 13 PP cultures obtained using 10 mM nicotinamide (ae, optimal differentiation) and 3.3 mM nicotinamide (fj, suboptimal differentiation) during stage 4 of differentiation. Cells were stained with anti-GP2 and anti-CD142 and then fixed and stained for the intracellular markers PDX1 and NKX6-1. IgG controls are shown on the left of each panel. Green boxes highlight the low percentage of GP2+/PDX− cells (ch), red box highlights the presence of CD142+PDX1− cells (j). Abbreviations: GP2, pancreatic secretory granule membrane major glycoprotein 2; CD142, tissue factor; NKX6-1, NK6 homeobox 1; PDX1, pancreatic, and duodenal homeobox 1

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