Fig. 2 | Nature Communications

Fig. 2

From: Endothelial adenosine A2a receptor-mediated glycolysis is essential for pathological retinal angiogenesis

Fig. 2

Endothelial Adora2a deficiency significantly decreases formation of pathological neovascularization in OIR retinas. a, b Quantification of pathological neovascularization and vessel dropout area (within the white borders) in postnatal day (P)17 OIR retinas. Adora2a flox/flox (Adora2a WT), Adora2a flox/floxLysmcre/cre (Adora2a Mφ-KO), and Adora2a flox/floxCdh5cre (Adora2a VEC-KO) mice were exposed to 70% O2 a or 75% O2 b. Areas of pathological neovascularization and vessel dropout are quantified as percentage of total retinal area. n = 16, 20, 15 retinas for a; n = 13, 10, 12 retinas for b; ***P < 0.001 vs. Adora2a WT group. Scale bars: 1000 μm. c Histological analysis of infiltration of neovascular nuclei from inner limiting membrane into vitreous in the OIR retinas. Nuclei on the vitreal side of the inner limiting membrane are indicated by asterisk. Scale bars: 100 μm. d Quantitative analysis of the number of neovascular nuclei in the OIR retinas. *P < 0.001 (n = 6 mice for Adora2a WT group; n = 8 for Adora2a Mφ-KO and Adora2a VEC-KO groups). e Ki-67 immunofluorescent staining on OIR retinas. Representative green (Ki-67), red (ERG), blue (nuclei, DAPI), and merged images were captured with confocal fluorescent microscopy. GCL ganglion cell layer, INL inner nuclear layer, ONL outer nuclear layer. Scale bars: 50 μm. f Quantitative analysis of the Ki-67 and ERG double-positive cells in each group. *P < 0.001 (n = 9 mice for each group). Data are represented as means ± s.e.m. Statistical significance was determined by one-way ANOVA followed by Bonferroni test