Fig. 1 | Nature Communications

Fig. 1

From: Endothelial adenosine A2a receptor-mediated glycolysis is essential for pathological retinal angiogenesis

Fig. 1

Localization and expression of Adora2a in rodent proliferative retinopathy. a Schematic illustration of mouse OIR model. Neonatal mice with nursing mothers were exposed to 75% O2 from postnatal day (P) 7 to P12, followed by room air (RA) with maximum neovascularization at P17. b Real-Time PCR analysis of Adora1, Adora2a, Adora2b, and Adora3 mRNA expression in the whole retina. Retinas were from RA or OIR mice at P17. ***P < 0.001 vs. RA group (n = 7 mice per group). c, d Real-Time PCR analysis of adenosine receptor mRNA expression in the whole retina. Retinas were obtained from mice at the times indicated. Data were normalized to both the expression of internal control and to gene mRNA expression of each RA control at each time point. **P < 0.01 vs. P12 (n = 4 mice per group for c and n = 7 mice per group for d). e, f Localization and expression of Adora2a in the RA and OIR retinas. Retinopathy was induced in wild-type mice. P17 RA and OIR retinas were stained with Adora2a (green), isolectin B4 (Lectin, red, vessel, e), or IBa1 (red, macrophages/microglias, f) and DAPI (blue, nuclei). In all, 2nd and 4th rows are magnification of the boxed regions in the 1st and 3rd rows, respectively. Scale bar: 50 μm (1st and 3rd rows) and 20 μm (2nd and 4th rows). g Real-Time PCR analysis of Adora2a mRNA expression in laser-capture microdissected pathological neovessels (tufts) from OIR mice compared with normal vessels from control mice raised in RA at P17. ***P < 0.001 vs. RA (n = 4 per group). Data are represented as means ± s.e.m. Statistical significance was determined by unpaired Student’s t-test (for b, g) and one-way ANOVA followed by Bonferroni test (for c, d)