Fig. 9 | Nature Communications

Fig. 9

From: pGlyco 2.0 enables precision N-glycoproteomics with comprehensive quality control and one-step mass spectrometry for intact glycopeptide identification

Fig. 9

Example of an O-glycopeptide SCE-HCD-MS/MS spectrum. A high-quality O-glycopeptide spectrum with abundant glycan and peptide fragment ions derived from stepped-energy HCD-MS/MS is shown. The design of the annotation in each spectrum is the same as that in Fig. 1. This O-glycopeptide has two potential glycosylation sites: T1 and S8. The three diagnostic ions with a HexNAc attached show that S8 is the glycosylation site: y9 + HexNAc (m/z = 1082.59, 1 + ), y11 + HexNAc (m/z = 1267.67, 1 + ), and y12 + HexNAc (m/z = 1366.73, 1+). A zoom-in figure of the latter two fragments is shown in the upper right corner of the spectrum annotation

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