Fig. 6 | Nature Communications

Fig. 6

From: pGlyco 2.0 enables precision N-glycoproteomics with comprehensive quality control and one-step mass spectrometry for intact glycopeptide identification

Fig. 6

Results of a large-scale intact N-glycopeptide analysis of mouse tissues. a Precursor mass deviation of 79,930 GPSMs. b Retention times of 61 different glycopeptides with the same peptide backbone ‘NLSYEAAPDHK’. The x-axis represents the window of retention time, the y-axis represents the log10 (intensity), and each color represents a different glycopeptide. c Example of a chimera spectrum from multiple glycopeptides. pGlyco 2.0 identified two different glycopeptides in one spectrum, illustrated in the form of a mirrored spectral annotation. The top and bottom spectra in each figure are the same spectrum with different glycopeptide identifications. The design of the annotation in each spectrum is the same as that in Fig. 1. d The MS1 data corresponds to the chimera spectrum