Fig. 5 | Nature Communications

Fig. 5

From: pGlyco 2.0 enables precision N-glycoproteomics with comprehensive quality control and one-step mass spectrometry for intact glycopeptide identification

Fig. 5

FDR validation results. a, b Isotopic peak pairing analysis of different glycopeptide identifications by pGlyco 2.0 and Byonic for the same spectrum. Both 15N- and 13C-labeled precursors agreed with the glycopeptides reported by pGlyco 2.0 a. Analysis of the heavily labeled precursor reported by Byonic in MS1. Neither the 15N- nor 13C-labeled precursors agreed with the glycopeptides reported by Byonic b. c FDRs of pGlyco 2.0 and Byonic for three LC-MS/MS runs. d FDR analysis of the glycopeptide identifications reported by Byonic under different score thresholds