Fig. 4 | Nature Communications

Fig. 4

From: Macrophage-released ADAMTS1 promotes muscle stem cell activation

Fig. 4

Adamts1 mice have increased satellite cell activity in response to injury. a (left) Images from IHC performed on histological sections prepared from injured TA muscles of 1-month-old Wt and Tg mice. EdU was injected into the mice at the time of muscle injury and mice were sacrificed for IHC on day 1. Histological sections were stained with DAPI (blue) to identify cell nuclei and EdU (green) to distinguish proliferating satellite cells. Scale bar = 100 μm. a (right) Quantification of the number of proliferating satellite cells after a muscle injury in Wt (n = 3) and Tg (n = 3) (>200 myofibers per mouse were examined). b (left) Images from IHC performed on histological sections prepared from injured TA muscle from Wt mice that received bone marrow transplantations from Wt mice (Wt-BMT) or Adamts1 mice (Tg-BMT) donors. Scale bar = 50 μm. b (right) Quantification of the number of proliferating satellite cells after a muscle injury in Wt-BMT (n = 3) and Tg-BMT (n = 4) mice (>200 myofibers per mouse were examined). *P < 0.05, ***P < 0.001. Error bars represent s.d. Statistical significance tested using paired t-tests

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