Fig. 3 | Nature Communications

Fig. 3

From: Macrophage-released ADAMTS1 promotes muscle stem cell activation

Fig. 3

Overexpression of Adamts1 promotes satellite cell activation in vivo. a Images from immunocytochemistry (ICC) performed on satellite cells immediately following isolation by FACS from wild-type (Wt) and Adamts1 (Tg) mice that had received an in vivo pulse of EdU. Cells were stained with DAPI (blue) to identify nuclei and EdU (green) and MyoD (red) to distinguish activated satellite cells (scale bar = 100 μm). b Quantification of the percent of satellite cells that are MyoD (left) and EdU (right) positive in Wt (n = 6) and Tg (n = 5) mice. c (upper) Images from IHC performed on histological sections prepared from TA muscles from Wt and Tg animals that had received an in vivo pulse of EdU and stained for DAPI (blue), EdU (green and arrows) and Dystrophin (white). Scale bar = 100 μm. (lower) Higher power magnification images of IHC showing EdU-positive cells (green) located beneath the myofiber membrane as delineated by Dystrophin (white) staining. d Quantification of the number of EdU-positive myonuclei per myofiber in Wt (n = 3) and Tg (n = 3) mice (>200 myofibers per mouse were examined). e Gross wet weights of tibialis anterior (TA) and quadriceps (Quad) muscles in Wt (n = 3) and Adamts1 transgenic (Tg; n = 4) mice at 1 month of age. f Histological sections prepared from TA muscles of 1-month-old mice and stained with hematoxylin and eosin (H&E). Scale bar = 50 μm. g Quantification of the diameters of myofibersin TA muscles from 1-month-old Tg (n = 4) and Wt (n = 3) mice. *P < 0.05, **P < 0.01. Error bars represent s.d. Statistical significance tested using paired t-tests

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