Fig. 5 | Nature Communications

Fig. 5

From: DNA methylation at enhancers identifies distinct breast cancer lineages

Fig. 5

DNA methylation of ERα, FOXA1 and GATA3 binding regions and expression of their target genes in Cluster 2. ac Average DNA methylation of CpGs in Cluster 2 and ERα a, FOXA1 b and GATA3 c binding regions defined by ChiP-seq peaks. Boxplots represent the average DNA methylation of these CpGs in ER positive (blue, n = 418), ER-negative tumors (red, n = 124) and adjacent normal tissue (green, n = 97). The average methylation of the Cluster2-CpGs in a TF binding site was significantly lower in ER positive patients compared to ER-negative and adjacent normal tissue. df Average gene expression of TF target genes in Cluster 2. d Estrogen (GRO-seq), e FOXA1 (siRNA) and f GATA3 (siRNA). Boxplots represent the average expression of the TF target genes in ER positive tumors (blue, n = 406), ER-negative tumors (red, n = 117) and adjacent normal tissue (green, n = 61). The average expression was significantly higher in ER positive tumors compared to ER-negative and adjacent normal tissue. Kruskal–Wallis test p-values are denoted

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