Table 3 Values of the coupling energy of Ser84 in E9 with Asn34 in Im2

From: Measuring inter-protein pairwise interaction energies from a single native mass spectrum by double-mutant cycle analysis

Ratio 1:1:1:1 1:2:2:1 1:1:2:1
Charge state +8 +9 +8 +9 +8 +9
ΔΔG int(ij) (kcal mol−1) −0.03 ± 0.02 −0.04 ± 0.04 0.01 ± 0.02 −0.02 ± 0.04 −0.07 ± 0.04 −0.08 ± 0.05
  1. Values of the coupling energy, ΔΔG int(ij), were calculated for the charge states +8 and +9 using the concentration ratios 1:1:1:1, 1:2:2:1, and 1:1:2:1 as defined in Fig. 3. Similar coupling energies are obtained for the different concentration ratios