Fig. 5 | Nature Communications

Fig. 5

From: Measuring inter-protein pairwise interaction energies from a single native mass spectrum by double-mutant cycle analysis

Fig. 5

Isothermal calorimetric titration of E9 endonuclease by an Im2 mutant. Shown is a representative experiment in which wild-type E9 endonuclease was titrated with the Asn34→Ala mutant of the Im2 immunity protein. The titration was carried out in 250 mM ethylenediammonium diacetate buffer (pH 7.0) at 25 °C as described under “Methods” section

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