Fig. 4 | Nature Communications

Fig. 4

From: RORα controls hepatic lipid homeostasis via negative regulation of PPARγ transcriptional network

Fig. 4

RORα interacts with HDAC3 to repress PPARγ transcriptional activity. a, b Effect of overexpression of RORα on PPRE-luciferase reporter activity with coactivator PGC1α a or NCOA2 b. *P < 0.05, **P < 0.01, ***P < 0.001, NS, non-significant, compared to PPARγ/coactivator group. c Effect of knockdown of RORα on PPRE-luciferase reporter activity. Cells were treated with DMSO (vehicle), rosiglitazone (20 μM) for 24 h. *P < 0.05, **P < 0.01, compared to shNS group. d Effect of RORα ΔDBD mutant on PPRE-luciferase reporter activity. ***P < 0.001. e Co-immunoprecipitation assay was performed to detect the interaction between RORα and HDACs of HEK293T cells. f Effect of RORα on PPRE-luciferase reporter activity by HDAC3 overexpression. *P < 0.05, **P < 0.01, compared to PPARγ/PGC1α group. g, h Effect of knockdown of HDAC3 with coactivator PGC1α g and NCOA2 h on PPRE-luciferase reporter activity. Data expressed as mean ± s.e.m. Statistical analysis was performed using one-way ANOVA followed by Tukey’s post hoc analysis. *P < 0.05, **P < 0.01. Data expressed as mean ± s.e.m

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