Fig. 2 | Nature Communications

Fig. 2

From: RORα controls hepatic lipid homeostasis via negative regulation of PPARγ transcriptional network

Fig. 2

Liver-specific Rorα deleted mice are susceptible to diet-induced hepatic steatosis and insulin resistance. aj RORαf/f and RORαLKO mice were fed with HFD for 10 weeks. a Representative liver histological section images of RORαf/f and RORαLKO mice stained with hematoxylin and eosin. Scale bar, 100 μm. b Macroscopic view of livers from RORαf/f and RORαLKO mice. c Liver weights of RORαf/f and RORαLKO mice (n = 10–11 per group). d Representative liver histological section images of RORαf/f and RORαLKO mice stained with Oil Red O. Scale bar, 100 μm. e Triglyceride content of livers from RORαf/f and RORαLKO mice (n = 8 per group). f Hepatic gene expression profile involved in metabolism from the livers of RORαf/f and RORαLKO mice (n = 4 per group) as determined by quantitative PCR with reverese transcription (qRT-PCR). Expression was normalized to 36B4 expression. g Fasting insulin levels in RORαf/f and RORαLKO mice (n = 6–7 per group). Data expressed as mean ± s.e.m. Statistical analysis was performed using Student’s unpaired t-test. *P < 0.05, **P < 0.01, ***P < 0.001, NS = non-significant. h Immunoblot analysis was performed from liver extracts of RORαf/f and RORαLKO mice. i, j Glucose tolerance test i and insulin tolerance test j on RORαf/f and RORαLKO mice fed on CD or HFD for 10 weeks. (n = 4–9/group). Data expressed as mean ± s.e.m. Statistical analysis was performed using Student’s unpaired t-test. *P < 0.05, RORαf/f vs RORαLKO, HFD. Data expressed as mean ± s.e.m

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