Fig. 1 | Nature Communications

Fig. 1

From: DELAY OF GERMINATION1 requires PP2C phosphatases of the ABA signalling pathway to control seed dormancy

Fig. 1

The YFP-DOG1 transgene complements the dog1-1 non-dormant phenotype. a Germination of freshly harvested seeds on 0.05% ethanol (control) or on 0.05% ethanol plus the indicated concentrations of fluridone. Radicle emergence was scored 7 days after sowing. Shown are averages  ± s.d. of three independent batches of seeds for each genotype. b YFP-DOG1 protein accumulation in the complementation lines. (top) Ponceau S staining. DOG1 protein was detected using GFP antibody. After stripping, the same membrane was blotted with H3 antibody as loading control. Cdog1#1-4 represent four independent dog1-1 transformants containing the YFP-DOG1 transgene

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