A Japanese boy with NAA10-related syndrome and hypertrophic cardiomyopathy

NAA10-related syndrome is an extremely rare X-chromosomal disorder, the symptoms of which include intellectual disability (ID), ocular anomalies, or congenital heart diseases, such as hypertrophic cardiomyopathy (HCM). Here, we describe a 4-year-old Japanese male patient who exhibited mild ID, HCM, and specific facial features. A hemizygous mutation (NM_003491.3: c.455_458del, p. Thr152Argfs*6) in exon 7 of NAA10 was detected. We recommend that patients undergo precise medical follow-up considering the characteristics of NAA10-related syndrome.

N-alpha-acetylation (N-terminal acetylation, NTA) is one of the most common protein modifications in eukaryotes, and~80% of the N-termini of human proteins are acetylated 1 . NAA10 (Xq28) encodes the enzyme N-alphaacetyltransferase 10 (NAA10), which is the catalytic subunit of the N-terminal acetyltransferase A complex with the protein encoded by NAA15 (4q31.1) 2 . NTA is essential for the preservation of normal cell function. However, its physiological significance has not been completely elucidated.
NAA10 abnormality in humans was originally known as causing Ogden syndrome (MIM #300855), which is an Xchromosomal inherited disorder. Female patients with Ogden syndrome show mild to severe intellectual disability (ID), and male patients die early in life 3 . Recently, several male patients with various NAA10 mutations have been reported to survive. These patients exhibit various phenotypes, such as hypertrophic cardiomyopathy (HCM) 4 , microphthalmia/anophthalmia 5 , or severe nonsyndromic ID 6 . These diseases due to NAA10 abnormalities are collectively called NAA10-related syndrome 1 . We report a Japanese boy carrying a hemizygous NAA10 mutation with HCM and ID but no microphthalmia.
Our patient was a Japanese boy who was the first child of healthy parents. He was delivered by elective cesarean section at 37 weeks of gestation because of a transverse position, and his Apgar score was 8/8 (at 1 and 5 min). His birth weight was 2170 g (small for gestational age). He exhibited genital abnormalities (split scrotum, hypospadias), eyelid drooping, and bilateral overlap of toes at birth. He was diagnosed with congenital heart disease (CHD) by echocardiography. His karyotype was 46,XY.
At 1 month of age, he was referred to our hospital for examination for CHD. Echocardiogram revealed a perimembranous outlet ventricular septal defect (defect: 5.0 mm × 4.5 mm), an atrial septal defect (defect: 3.5 mm × 3.6 mm), and left ventricle (LV) wall thickness (interventricular septum: 5.4 mm; left ventricular posterior wall: 3.7 mm) (Fig. 1a). At the age of 5 months, echocardiogram showed thickening of the LV, especially the interventricular septum. Thus, he was diagnosed with HCM with LV outflow tract obstruction (LVOTO) and trivial-mild mitral regurgitation (MR). After 1 month, the MR and LVOTO worsened, and β-blocker therapy was started.
At the age of 1 year, he was referred to the Clinical Genetics Department for molecular diagnosis. He showed mild ID, characteristic facial features, including eyelid drooping, exophthalmos, underdeveloped superior crus of antihelix, unilateral hearing loss, bifid scrotum, hypospadias, perodactylia, and CHD. His height at the age of 1 year was 69.6 cm (−2.0 SD), and his body weight was 8.4 kg (body mass index (BMI) 17.3). Routine blood tests and serum amino acid analysis were normal. He started to walk independently at the age of 1 year and 7 months. At the age of 4 years, his height was 90.0 cm (−2.63 SD), and his body weight was 12.2 kg (BMI 15.1). He was able to trot around, but he was unable to speak meaningful words. Brain magnetic resonance at the age of 1 year and 6 months imaging did not show any abnormalities.
To confirm his molecular diagnosis, we analyzed DNA samples derived from his peripheral blood by nextgeneration sequencing using TruSight One (Illumina, San Diego, CA, USA) after obtaining written informed consent from his parents. All procedures were reviewed and approved by the Institutional Review Board of Kobe University School of Medicine (86) and Hyogo Prefectural Kobe Children's Hospital (28-4) and were in accordance with the ethical standards of the Declaration of Helsinki. We identified a hemizygous four-base deletion (NM_003491.3: c.455_458del, p. Thr152Argfs*6) in exon 7 of NAA10. The same deletion was identified in his asymptomatic mother, although in the heterozygous state (Fig. 1b). Other pathogenic variants, including genes associated with RASopathies, were not identified by TruSight One.
Male patients with NAA10-related syndrome are extremely rare and present various clinical features. Previously, it was considered that boys with NAA10 mutations die early in life 2 . To our knowledge, 35 male patients from 13 families with 11 types of genetic aberrations in NAA10 have been reported ( Table 1). The frequently observed symptoms are as follows: Fig. 1 The results of the cardiac and genetic testing in the patient. a Echocardiography of the patient at 1 year of age. b NAA10 mutations were identified in the patient and his mother by the Sanger method. Red letters indicate deleted nucleotides. c The genotype of the NAA10 mutation in male patients of the present case and literature. The bold face indicates hypertrophic myopathy, and the dotted line shows microphthalmia or anophthalmia. Table 1 Phenotypes and genotypes of male patients with NAA10-related syndrome.
Author Rope et al. 3 Casey et al. 8 Støve et al. 4 Saunier et al. 9 Ree et al. 10 Popp et al. 6 Esmailpour et al. 5 Cheng et al. 2 Slavotinek and Lee 11 , Johnston et al. 7 Johnston et al. 7 Johnston et al. 7 The present case  ID, motor developmental delay, growth failure, ophthalmic diseases, skeletal disorders, including scoliosis or digital anomalies, and cardiac disorders. Most NAA10 mutation types in male patients are missense variants, but three families, including the present case, harbor truncated mutations 2,7 . Patients with NAA10 mutations in further unstructured domains (exons 7 or 8) tend to exhibit microphthalmia or anophthalmia (Fig. 1c). The mutation of the present patient is a frameshift mutation that is identical to that of a boy reported by Cheng 3 . We presumed that he survived because the mutation is in a region where nonsensemediated degradation does not occur. However, our patient did not show microphthalmia/anophthalmia. Further investigations are needed to clarify the precise mechanism of microphthalmia in NAA10-related syndrome. Cardiac complications are also observed in male patients with NAA10related syndrome. However, the genotype-phenotype correlation is not clear. In addition, the exact cause of HCM in the present patient remains unknown. Female patients with NAA10-related syndrome may display various types of ID 6 . The mother of the present patient carried an identical NAA10 mutation in the heterozygous state, but she did not exhibit any medical abnormalities. Families with NAA10-related syndrome should be considered during genetic counseling with regard to recurrence in the next child, irrespective of the sex of the child.

Gender
In conclusion, we report for the first time a Japanese male patient with NAA10-related syndrome. We recommend that patients undergo precise medical follow-up, particularly for neurodevelopment, cardiac disease, including HCM, ocular abnormalities, and scoliosis. The results of our study are useful for the recognition of NAA10-related syndrome.

HGV database
The relevant data from this Data Report are hosted at the Human Genome Variation Database at https://doi.org/10.6084/m9.figshare.hgv.2879.