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Mapping of quantitative trait loci for life history traits segregating within common frog populations

Heredity (2019) | Download Citation

Abstract

The evolution of complex traits is often shaped by adaptive divergence. However, very little is known about the number, effect size, and location of the genomic regions influencing the variation of these traits in natural populations. Based on a dense linkage map of the common frog, Rana temporaria, we have localized, for the first time in amphibians, three significant and nine suggestive quantitative trait loci (QTLs) for metabolic rate, growth rate, development time, and weight at metamorphosis, explaining 5.6–18.9% of the overall phenotypic variation in each trait. We also found a potential pleiotropic QTL between development time and size at metamorphosis that, if confirmed, might underlie the previously reported genetic correlation between these traits. Furthermore, we demonstrate that the genetic variation linked to fitness-related larval traits segregates within Rana temporaria populations. This study provides the first insight into the genomic regions that affect larval life history traits in anurans, providing a valuable resource to delve further into the genomic basis of evolutionary change in amphibians.

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Acknowledgements

We are indebted to Cristina García, Leticia Viesca, and Antonio Sánchez-Palacio for helping with tadpole care and phenotypic measurements, Sara Knott and D.J. de Koning for assistance with data analysis and software, and Jaime Bosch and Miguel Tejedo for useful suggestions. We are grateful to the two anonymous reviewers for improving this article significantly with their contribution. We thank the Government of Castilla y León and Cantabria for providing us the permit to conduct this investigation. This research was supported by the following grants: Spanish Ministry of Education (references CGL2011-23443 and CGL2012-40246-C02-02), Ministry of Economy and Competitiveness (reference BES-2012-055220), National Parks Autonomous Agency (OAPN) (reference MARM 428/211), Estonian Ministry of Education and Research (institutional research funding project IUT8-2), and the Academy of Finland (Grant No. 266321).

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Affiliations

  1. Research Unit of Biodiversity (UO-CSIC-PA), 33600, Mieres, Asturias, Spain

    • Gemma Palomar
    • , Alfredo G. Nicieza
    •  & José Manuel Cano
  2. Department of Biology of Organisms and Systems, University of Oviedo, 33006, Oviedo, Asturias, Spain

    • Gemma Palomar
    • , Alfredo G. Nicieza
    •  & José Manuel Cano
  3. Molecular and Behavioral Ecology Group, Institute of Environmental Sciences, Jagiellonian University, 30-387, Krakow, Poland

    • Gemma Palomar
  4. Department of Biology, University of Turku, 20014, Turku, Finland

    • Anti Vasemägi
    •  & Freed Ahmad
  5. Department of Aquaculture, Institute of Veterinary Medicine and Animal Science, Estonian University of Life Sciences, 51006, Tartu, Estonia

    • Anti Vasemägi
  6. Department of Aquatic Resources, Institute of Freshwater Research, Swedish University of Agricultural Sciences, 17893, Drottningholm, Sweden

    • Anti Vasemägi

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The authors declare that they have no conflict of interest.

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Correspondence to Gemma Palomar.

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https://doi.org/10.1038/s41437-018-0175-x