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Understanding the evolutionary potential of epigenetic variation: a comparison of heritable phenotypic variation in epiRILs, RILs, and natural ecotypes of Arabidopsis thaliana

Heredityvolume 121pages257265 (2018) | Download Citation

Abstract

Increasing evidence for epigenetic variation within and among natural plant populations has led to much speculation about its role in the evolution of plant phenotypes. However, we still have a very limited understanding of the evolutionary potential of epigenetic variation, in particular in comparison to DNA sequence-based variation. To address this question, we compared the magnitudes of heritable phenotypic variation in epigenetic recombinant inbred lines (epiRILs) of Arabidopsis thaliana—lines that mainly differ in DNA methylation but only very little in DNA sequence—with other types of A. thaliana lines that differ strongly also in DNA sequence. We grew subsets of two epiRIL populations with subsets of two genetic RIL populations, of natural ecotype collections, and of lines from a natural population in a common environment and assessed their heritable variation in growth, phenology, and fitness. Among-line phenotypic variation and broad-sense heritabilities tended to be largest in natural ecotypes, but for some traits the variation among epiRILs was comparable to that among RILs and natural ecotypes. Within-line phenotypic variation was generally similar in epiRILs, RILs, and ecotypes. Provided that phenotypic variation in epiRILs is mainly caused by epigenetic differences, whereas in RILs and natural lines it is largely driven by sequence variation, our results indicate that epigenetic variation has the potential to create phenotypic variation that is stable and substantial, and thus of evolutionary significance.

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Acknowledgements

We are grateful to Vincent Colot and Jurek Paszkowski for providing seed materials of epiRILs, and for their helpful suggestions on the manuscript. This work was supported by the Swiss National Science Foundation [grant no. 31EE30-131171 to O.B.] as part of the European Science Foundation EUROCORES Programme EuroEEFG, the National Natural Science Foundation of China [grant no. 31600291 to Y-Y.Z.], and the Fundamental Research Funds for the Central Universities in China [grant no. 20720170074 to Y-Y.Z.].

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Affiliations

  1. Institute of Plant Sciences, University of Bern, CH-3013, Bern, Switzerland

    • Yuan-Ye Zhang
    • , Vit Latzel
    • , Markus Fischer
    •  & Oliver Bossdorf
  2. Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, College of the Environment and Ecology, Xiamen University, Xiamen, Fujian, 361102, China

    • Yuan-Ye Zhang
  3. Institute of Botany of the ASCR, CZ-252 43, Průhonice, Czech Republic

    • Vit Latzel
  4. Plant Evolutionary Ecology, University of Tübingen, D-72076, Tübingen, Germany

    • Oliver Bossdorf

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The authors declare that they have no conflict of interest.

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Correspondence to Yuan-Ye Zhang.

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https://doi.org/10.1038/s41437-018-0095-9

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