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Natural re-colonization and admixture of wolves (Canis lupus) in the US Pacific Northwest: challenges for the protection and management of rare and endangered taxa

Heredity (2018) | Download Citation

Abstract

Admixture resulting from natural dispersal processes can potentially generate novel phenotypic variation that may facilitate persistence in changing environments or result in the loss of population-specific adaptations. Yet, under the US Endangered Species Act, policy is limited for management of individuals whose ancestry includes a protected taxon; therefore, they are generally not protected under the Act. This issue is exemplified by the recently re-established grey wolves of the Pacific Northwest states of Washington and Oregon, USA. This population was likely founded by two phenotypically and genetically distinct wolf ecotypes: Northern Rocky Mountain (NRM) forest and coastal rainforest. The latter is considered potentially threatened in southeast Alaska and thus the source of migrants may affect plans for their protection. To assess the genetic source of the re-established population, we sequenced a ~ 300 bp portion of the mitochondrial control region and ~ 5 Mbp of the nuclear genome. Genetic analysis revealed that the Washington wolves share ancestry with both wolf ecotypes, whereas the Oregon population shares ancestry with NRM forest wolves only. Using ecological niche modelling, we found that the Pacific Northwest states contain environments suitable for each ecotype, with wolf packs established in both environmental types. Continued migration from coastal rainforest and NRM forest source populations may increase the genetic diversity of the Pacific Northwest population. However, this admixed population challenges traditional management regimes given that admixture occurs between an adaptively distinct ecotype and a more abundant reintroduced interior form. Our results emphasize the need for a more precise US policy to address the general problem of admixture in the management of endangered species, subspecies, and distinct population segments.

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Acknowledgements

We are grateful to Kristin Mansfield and Roblyn Brown for samples and insight into Washington and Oregon wolves, respectively. Laboratory and data processing assistance was completed by Sarah Wenner, Daniel Greenfield and Jacqueline Robinson. We thank Brendan Epstein for helpful discussions concerning methods' implementation and Kim Andrews for discussions regarding data interpretation within this study. This work was supported by the Wilburforce Foundation and the National Science Foundation (NSF; DEB-1021397, OPP-0733033). Additional support was provided to SAH by the National Institute of Health (P30GM103324), NSF (DEB-1316549) and the Bioinformatics and Computational Biology program at the University of Idaho; to RMS by the NSF (DGE-1144087, DGE-0707424, 1612859); and to RJH by the NSF (PD-08-1269). This work used the Vincent J. Coates Genomics Sequencing Laboratory at UC Berkeley, supported by NIH (S10RR029668, S10RR027303).

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  1. These authors contributed equally: Sarah A. Hendricks, Rena M. Schweizer.

Affiliations

  1. Department of Biological Sciences and Institute of Bioinformatics and Evolutionary Studies, University of Idaho, 875 Perimeter Drive, Moscow, ID, 83844, USA

    • Sarah A. Hendricks
    •  & Paul A. Hohenlohe
  2. Division of Biological Sciences, University of Montana, 32 Campus Drive, Missoula, MT, 59812, USA

    • Rena M. Schweizer
  3. Center for Tropical Research, Institute of the Environment and Sustainability, University of California, Los Angeles, 300 LaKretz Hall, Los Angeles, CA, 90095, USA

    • Ryan J. Harrigan
    •  & John P. Pollinger
  4. Department of Ecology and Evolutionary Biology, University of California, Los Angeles, 610 Charles E. Young Drive East, Los Angeles, CA, 90095, USA

    • John P. Pollinger
    •  & Robert K. Wayne
  5. Raincoast Conservation Foundation, Sidney, BC, V8L 3Y3, Canada

    • Paul C. Paquet
    •  & Chris T. Darimont
  6. Department of Geography, University of Victoria, Box 1700, Stn CSC, Victoria, BC, V8W 2Y2, Canada

    • Paul C. Paquet
    •  & Chris T. Darimont
  7. Department of Fish and Wildlife Sciences, University of Idaho, 875 Perimeter Drive, Moscow, ID, 83844, USA

    • Jennifer R. Adams
    •  & Lisette P. Waits
  8. Department of Ecology and Evolutionary Biology, Princeton University, M151 Guyot Hall, Princeton, NJ, 08544, USA

    • Bridgett M. vonHoldt

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https://doi.org/10.1038/s41437-018-0094-x