Fine-scale temperature-associated genetic structure between inshore and offshore populations of sea scallop (Placopecten magellanicus)

Abstract

In the northwest Atlantic Ocean, sea scallop (Placopecten magellanicus) has been characterized by a latitudinal genetic cline with a breakpoint between northern and southern genetic clusters occurring at ~45°N along eastern Nova Scotia, Canada. Using 96 diagnostic single-nucleotide polymorphisms (SNPs) capable of discriminating between northern and southern clusters, we examined fine-scale genetic structure of scallops among 27 sample locations, spanning the largest geographic range evaluated in this species to date (~37–51°N). Here, we confirmed previous observations of northern and southern groups, but we show that the boundary between northern and southern clusters is not a discrete latitudinal break. Instead, at latitudes near the previously described boundary, we found unexpected patterns of fine-scale genetic structure occurring between inshore and offshore sites. Scallops from offshore sites, including St. Pierre Bank and the eastern Scotian Shelf, clustered with southern stocks, whereas inshore sites at similar latitudes clustered with northern stocks. Our analyses revealed significant genetic divergence across small spatial scales (i.e., 129–221 km distances), and that spatial structure over large and fine scales was strongly associated with temperature during seasonal periods of thermal minima. Clear temperature differences between inshore and offshore locations may explain the fine-scale structuring observed, such as why southern lineages of scallop occur at higher latitudes in deeper, warmer offshore waters. Our study supports growing evidence that fine-scale population structure in marine species is common, often environmentally associated, and that consideration of environmental and genomic data can significantly enhance the identification of marine diversity and management units.

Introduction

Many marine species are characterized by a planktonic larval stage, and, given few conspicuous barriers to dispersal in the ocean, the potential for connectivity among populations across broad spatial scales may be significant (Cowen and Sponaugle, 2009; Hellberg, 2009). While patterns of weak genetic structure across large geographic ranges are found in some marine species (Gaither et al. 2011; Simpson et al. 2014; Yahagi et al. 2017), these cases may represent exceptions rather than the rule (Hauser and Carvalho 2008). Advances in marine genetics and genomics are increasingly revealing that, despite high dispersal potential, fine-scale genetic structure does occur within many marine species (Knutsen et al. 2007; Selkoe et al. 2008; Benestan et al. 2015; Gagnaire et al. 2015).

Fine-scale genetic structure in marine systems has been attributed to a combination of oceanographic and biological processes often influencing larval dispersal (Stanley et al. 2012; Treml et al. 2015), such as complex oceanographic features and larval behavior (Taylor and Hellberg, 2003; Jones et al. 2005; Knutsen et al. 2007; Bradbury et al. 2008; Teske et al. 2015). Oceanographic currents can move larvae long distances (Simpson et al. 2014), or complex oceanographic circulation patterns can retain larvae producing fine-scale genetic differentiation (i.e., Panulirus argus; Truelove et al. 2016). Similarly, larval behavior (i.e., vertical migration) can restrict dispersal and gene flow, thus leading to highly structured populations (Bradbury et al. 2008). However, in addition to processes that influence dispersal, recent evidence also suggests natural selection and/or intrinsic barriers to gene flow may constrain connectivity in the ocean (Clarke et al. 2010; Bradbury et al. 2013; Momigliano et al. 2017; Van Wyngaarden et al. 2017).

Adaptation to the local environment can result in genetic divergence over small spatial scales, where heterogeneous environments drive genetic differences between populations (Jørgensen et al. 2005; Bradbury et al. 2010; Milano et al. 2014; Silva et al. 2014; Benestan et al. 2016). For example, in Atlantic silverside (Menidia menidia), latitudinal genetic variation exists for several traits (e.g., temperature-dependent sex determination) despite significant dispersal among distant sites (Schultz and Conover, 1997; Schultz et al. 1998; Clarke et al. 2010; Duffy et al. 2015). Similarly, climate-associated clinal genetic variation has been observed in several species in the northwest Atlantic Ocean (Stanley et al. 2018) over fine spatial scales. Growing evidence of significant population structure counters widely held perceptions of panmixia in the marine environment, and represents a paradigm shift in marine ecology that has significant consequences for the management and conservation of marine species (Hauser and Carvalho, 2008; Reiss et al. 2009).

Sea scallop (Placopecten magellanicus Gmelin, 1791; Pectinidae: Bivalvia) is one of eastern North America’s most important commercial fisheries (Naidu and Robert, 2006), ranking third in the United States and fourth in Canada in terms of landed values ($440 million (USD) and $184 million (CAD); NOAA, 2016; DFO, 2016a). Sea scallops are native to the northwest Atlantic Ocean, and the species range extends from northern Newfoundland (NL), Canada, to Cape Hatteras in North Carolina, US (Posgay 1957). Generally, sea scallops are found on the continental shelf at depths ranging from 10 to 100 m where they form high-density benthic aggregations known as 'beds' (Naidu and Robert 2006). Although sea scallop adults are primarily sedentary with limited dispersive capacity (Melvin et al. 1985), they are highly fecund broadcast spawners that produce planktonic larvae that can remain in the water column for up to 40 days (Culliney 1974; Naidu and Robert 2006). Given their planktonic larval duration, it is likely that scallop larvae have extensive dispersal ability resulting in connectivity between stocks (Tian et al. 2009; Davies et al. 2014).

Despite relatively high dispersal potential, the genetic structure of sea scallops is characterized by two broadly defined genetic clusters (northern and southern clusters) with a genetic breakpoint occurring along the coast of eastern Nova Scotia (NS; Van Wyngaarden et al. 2017) potentially associated with environmental variation (Van Wyngaarden et al. 2018). Across the range, sea scallops occupy a variety of habitats encompassing gradients of depth, substrate, temperature, and ocean chemistry (Townsend et al. 2004; Naidu and Robert 2006; Van Wyngaarden et al. 2018). Broad-scale genetic–environment associations have been examined using a suite of environmental variables (e.g., temperature, salinity, seawater density, chlorophyll A, and inorganic nutrient concentrations; Van Wyngaarden et al. 2018). Colder temperatures (winter and annual minima) and to a lesser extent salinity were strongly associated with genetic variation, potentially implicating post-settlement selection as a driver of broad-scale structure (Van Wyngaarden et al. 2018). In addition to clinal structuring, some evidence exists that fine-scale genetic structure may be present within some regions such as the Gulf of Maine and Georges Bank (Kenchington et al. 2006; Owen and Rawson 2013).

In our study, the main objective is to explore the presence and fine-scale distribution of northern and southern genetic clusters of sea scallop and examine possible drivers of fine-scale structure. We build on previous genetic and genomic studies (Kenchington et al. 2006; Owen and Rawson, 2013; Van Wyngaarden et al. 2017, 2018) through increased sampling spanning the largest geographic range to date. We genotyped adult scallops from across the range utilizing a small panel of diagnostic (high FST with low linkage disequilibrium) single-nucleotide polymorphisms (SNPs) capable of discriminating between the northern and southern genetic clusters. In addition, given the potential role of cold temperatures on post-settlement survival (Van Wyngaarden et al. 2018), we genotyped pre- and post-winter juvenile scallops from a subset of locations to evaluate the role of selection in shaping genetic structure. Further, as previous evidence suggests that fine-scale genetic structure exists in parts of the sea scallop range (Kenchington et al. 2006; Owen and Rawson 2013), genotype data were analyzed in combination with salinity and temperature data to evaluate how environmental conditions potentially mediate fine-scale genetic structure, and thus provide valuable insight into the mechanisms driving genetic structure in an ocean characterized by few obvious physical barriers to gene flow. A more informed evaluation of genetic population structure in the economically significant sea scallop is necessary to ensure integration between management action and the biological units of interest.

Materials and Methods

Sample collection

Muscle tissue samples from sea scallop adults were collected from 24 sites across eastern North America from 2012 to 2015 (Table 1 and Fig. 1). In addition to adult samples, scallop spat (young-of-the-year) were collected from two of the adult locations (Passamaquoddy Bay, New Brunswick (NB) and Chester, NS) as well as three additional locations in NS (Arisaig, Chedabucto and St. Anne’s Bay; see Table 1 and Fig. 1). To examine the potential role of post-settlement selection in shaping genetic structure in sea scallop (Van Wyngaarden et al. 2018), four of the five spat-sampling locations in our study, were sampled both pre- (November/December 2015) and post-winter (May 2016; see Table 1). Overall, the 24 adult sites plus an additional three locations for spat sampling equated to a total of 27 different sites, and all tissue samples from these sites were preserved in AllProtect (Qiagen, Toronto, ON, Canada) or 80% ethanol.

Table 1 Sea scallop (Placopecten magellanicus) sampling site locations and codes with the number of individuals sampled for each life stage and sampling year
Fig. 1
figure1

Results of Bayesian clustering analysis for sea scallop (Placopecten magellanicus) sampling locations using 96 collectively diagnostic loci. a Individual assignment of scallops to two genetic clusters (north (blue) and south (red)) where each individual is represented by a vertical bar indicating the proportion of membership to each cluster. b Map of sampling locations with the proportion of membership assigned to two genetic clusters. The optimal number of clusters (K) was determined based on the delta K statistic of Evanno et al. 2005. Sites are overlaid on winter bottom temperatures averaged from 2011 to 2015, where color scale indicates temperature in degrees Celsius. Site abbreviations are listed in Table 1

DNA extraction and SNP genotyping

DNA was isolated from the tissue samples using DNeasy 96 Blood and Tissue kit (Qiagen, Toronto, ON Canada) following the protocol from the manufacturer, without optional RNase A (Qiagen) treatment. All DNA samples were quantified following the same protocol as described in Van Wyngaarden et al. (2017) using Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA) and the FLUOStar OPTIMA fluorescence plate reader (BMG Labtech, Ortenberg, Germany). A subset of scallops (n = 238) from 12 sites in our study (see Table 1) were previously genotyped at 7163 SNPs using restriction-site-associated DNA sequencing (RAD-seq; see Van Wyngaarden et al. 2017). Of the 7163 SNPs, a panel of diagnostic SNPs, comprising those with the highest FST that also showed low linkage disequilibrium, was created. During panel construction, candidate SNP Type assays (Fluidigm, San Francisco, CA, USA) were tested on a set of samples, which included samples that were used to generate the RAD-seq libraries as these would have known genotypes at the target loci (Van Wyngaarden et al. 2017). Loci were selected for inclusion in the final SNP panel based on the SNP Type assay results including (1) the cluster pattern being easy to interpret, (2) the generation of correct genotypes for known samples and positive controls (see below), (3) reproducible genotypes across multiple chip runs, and (4) the ranking of the target SNP in the prioritized list. The final panel consisted of 96 SNPs (see Table S1) and 20% of these SNPs were previously identified as environmentally associated outliers using one or more detection methods with a data set of 90 environmental variables (Van Wyngaarden et al. 2018). gBlocks (Integrated DNA Technologies, Coralville, IA, USA) were designed and synthesized for use as positive controls (Richards-Hrdlicka 2014).

A total of 1104 scallops from 27 locations (including individuals from 15 new sites and additional individuals from the same 12 sites from Van Wyngaarden et al. 2017) were genotyped using the SNP Type assays (Fluidigm) as per the manufacturer’s protocols with the STA (specific target amplification) step, using 96.96 genotyping integrated fluidic circuits and read on an EP1 (Fluidigm) and analyzed using SNP Genotyping Analysis software (Fluidigm). DNA samples had all been extracted previously, and each 96-well plate set-up included 10 samples that were repeated on the plate (redundants) to detect processing errors (row or plate reversal) and ensure consistent clustering interpretation. Samples below the genotype quality threshold (>9 failed loci) were dropped from the data set (34 samples in total; 3%). A subset of samples (8.6%) was reanalyzed, including re-extraction of DNA from the original tissue if available, in order to calculate the genotyping error rate. The genotype error rate was calculated based on Pompanon et al. 2005 to be 0.005. This is slightly higher than other reported studies (0–0.002; Larson et al. 2013; Petrou et al. 2013; Hess et al. 2015). After quality-checking and prior to genetic analyses, our Fluidigm genotype data (n = 1070) were combined with publicly available data (n = 238) from Van Wyngaarden et al. (2017) using genepopedit (Stanley et al. 2017).

Population genetic structure

Pairwise genetic divergence (FST) was calculated using ARLEQUIN v3.5 (Excoffier and Lischer 2010) for all 27 sites and all samples. Using all pairwise FST values between sampling sites, a heatmap was created using the R package gplots (Warnes et al. 2016). Next, population structure was examined using non-metric multidimensional scaling (nMDS) analysis based on population allele frequencies in vegan (Oksanen et al. 2017) and Bayesian clustering using the program STRUCTURE v2.3.4 (Pritchard et al. 2000). All STRUCTURE runs were implemented through the R package ParallelStructure (Besnier and Glover 2013) with K values tested ranging from 1 to 27 (i.e., total number of sites). For each value of K, three independent Markov Chain Monte Carlo (MCMC) runs were performed using 100,000 burn-in and 500,000 iterations. The delta K statistic described by Evanno et al. 2005 was used to determine the best K for the data set using STRUCTURE HARVESTER (Earl and vonHoldt 2012). Genetic clusters were visualized using CLUMPAK (Kopelman et al. 2015), and admixture coefficients (Q value) from STRUCTURE were used in subsequent analyses.

Next, spatial genetic relationships were further explored using spatial principal component analysis (SPCA; Jombart et al. 2008), which accounts for individual genetic variability while controlling for spatial autocorrelation (Jombart et al. 2008). SPCA was run in adegenet (Jombart 2008) with significance tests for global and local structure patterns based on 1000 permutations. Cartesian coordinates of sites were used to build a connection network using Delaunay triangulation (Upton and Fingleton 1985). Cartesian coordinates were determined in R software by reprojecting site coordinates in Cartesian space while accounting for land barriers using pairwise least-cost distances among sites calculated from NOAA bathymetry data in marmap (Pante and Simon-Bouhet 2013) and using nMDS analysis in vegan (Oksanen et al. 2017). SPCA PC axis 1 lagged scores were used to visualize differences among sites and across latitude, as these scores are generally used to better observe global structure (Jombart et al. 2008).

Genetic variation across life stage: examining the role of post-settlement selection

After examining the presence of genetic structure across the range, we next examined temporal genetic differences between samples collected from the same location using ARLEQUIN v3.5 (Excoffier and Lischer 2010), where pairwise genetic divergence (FST) was calculated between pre- and post-winter spat samples (four sites). Alpha level was adjusted to account for multiple comparisons (alpha = 0.05/4 = 0.0125). The same analyses were conducted to compare differences between adults and spat samples from the same location (two sites) to further examine the role of post-settlement selection on genetic structure. The genetic distances among life stages and site locations were also compared using a neighbor-joining tree based on Cavalli-Sforza and Edwards 1967 chord distances calculated in POPULATIONS v1.2.33 (Langella 2012) with 1000 bootstrap replicates. FigTree v1.4 (Rambaut 2012) was used to visualize the relationships among samples.

Environmental factors driving population structure

We selected averaged annual maximum and minimum, and seasonally averaged temperature and salinity from the sea surface and bottom for all scallop sites between 2011 and 2015. All 24 environmental measures were assembled at spatial resolutions interpretable to 1/12° (or ~5 km2) from a numerical model (NEMO) of the northwest Atlantic Ocean (Brickman et al. 2016). All data were converted to four seasonal climatological data (2011–2015) layers, of 3 months each, effectively corresponding with winter (January–March), spring (April–June), summer (July–September), and fall (October–December). Annual minimum and maximum temperature and salinity data layers were also estimated from the modeled temperature and salinity data. All resultant data layers covering the known distribution of sea scallop were converted to ASCII grid with WGS84 global stereographic projection and a uniform land mask applied. Values for each of these environmental variables, corresponding with each geo-referenced sample location, were subsequently extracted from the gridded temperature and salinity data layers using the extract by points tool in ArcMap for subsequent analyses.

To identify environmental variables associated with genetic variation, we used two different approaches: redundancy analysis (RDA) and random forest (RF) regressions. RDAs were performed using the R package vegan (Oksanen et al. 2017) where all 24 environmental variables (standardized) were used as constraining variables to explain allele frequencies across the 96 loci. First, the ordistep function, a stepwise permutational ordination method, was implemented in both directions (forward and backward) with 1000 permutations to select environmental variables that best explained the genetic variance. ANOVAs with 1000 permutations were used to evaluate the global significance of the RDA and marginal ANOVAs (1000 permutations) were used to evaluate the significance of each selected environmental variable. To partition the genetic variance explained by environment and space separately, we used partial RDA (pRDA) conditioned on geography (Cartesian coordinates) and pRDA conditioned on environment (selected variables) separately.

Multiple RF regressions (Breiman 2001) were run using the R package randomForest (Liaw and Wiener 2002) where allele frequencies for the 96 SNPs were used as individual response vectors and our 24 environmental variables (standardized) were used as the set of predictor variables. In the regressions, the number of parameters sampled for each node split (mtry) was selected using the tuneRF function in randomForest (Liaw and Wiener 2002). All other parameters were set to default with 10,000 trees for each run. To determine the relative importance of each environmental variable, the mean decrease in accuracy was averaged for each variable across runs and loci. Site-specific mean residual square error was also calculated across runs and loci.

Results

Population genetic structure

The heatmap of pairwise FST values revealed regions where genetic divergence was generally lower among sites across large distances as well as areas where genetic divergence was high between nearby sites (Fig. 2a). Sites within the southern region (south of 45°N in NS) generally showed lower genetic divergence with each other compared to other sites, where only 51 of the 153 pairwise comparisons were significant (p values < 0.002). In addition, FST was generally lower (but often significant) among sites in northern NS and the Gulf of St. Lawrence (Fig. 2a and Table S2). Sites in Newfoundland (NL) were significantly genetically divergent from each other (with the exception of Sunnyside, Trinity Bay (SUN) and Croque (CRQ); Fig. 2a and Table S2).

Fig. 2
figure2

a Heatmap showing pairwise genetic divergence (FST) below diagonal with corresponding significance (p values) above diagonal between all sea scallop (Placopecten magellanicus) sites ordered from south to north. Below the diagonal comparisons with greater divergence (high FST) are indicated in red, whereas more similar comparisons (low FST) are indicated in blue. Correspondingly above the diagonal, significant comparisons are indicated by red and nonsignificant (p ≥ 0.00185) comparisons are indicated by blue. b Non-metric multidimensional scaling (nMDS) analysis plot of sea scallop sampling sites based on allele frequencies at 96 diagnostic loci performed using vegan (Oksanen et al. 2017). Colors of site correspond to their location being north (blue) or south (red) of the previously described genetic breakpoint. Stress for nMDS projection was relatively low (0.12)

Bayesian clustering analysis revealed two genetic clusters (north and south) based on the delta K statistic of Evanno et al. 2005 (Fig. 1a,b). STRUCTURE showed that sites south of 45°N (along eastern NS) clustered together, with the exception of Chester, NS (CHE), which clustered with the northern sites. Sites north of 45°N were all one genetic cluster with the exception of St. Pierre Bank (STB), which clustered with the southern genetic group. Relationships were consistent with nMDS analyses (Fig. 2b).

Similar spatial genetic patterns were found when evaluated using SPCA (Fig. 3a). Site locations were controlled for in the SPCA using Cartesian distances between sites and these distances closely aligned with geographic distances between sites (R2Adj = 0.98, p < 0.001) with a relatively low stress for nMDS projection (0.035). SPCA revealed strong global structure (p < 0.001) corresponding to genetic differences between the northern and southern regions (Fig. 3a), and a clinal pattern was observed across latitude (Fig. 3b). However, SPCA also revealed significant local structure (p = 0.001) indicating fine-scale genetic structure between nearby sites (Fig. 3a). STB again showed a more southern signature (i.e., lower SPCA axis 1 lagged score) differing from the nearby LTB site (Fig. 3a). In addition, sites located in close proximity spatially (offshore and inshore sites in NS) showed different genetic signatures where inshore CDB differed from nearby offshore eastern Scotian Shelf sites (SSM, SSQ, and SSW), and similarly inshore CHE differed from these sites as well as nearby sites including SFA, SSB, and SSG (Fig. 3a).

Fig. 3
figure3

a Sea scallop (Placopecten magellanicus) sampling sites plotted based on their relative spatial position based on Cartesian coordinates. Sites are overlaid on colors corresponding to their relative genetic grouping based on axis 1 lagged scores of the spatial principal component analysis (SPCA), where red represents more southern genotypes and blue represents more northern genotypes. b Standardized SPCA axis 1 lagged scores for all sea scallops plotted against latitude for the sampling site demonstrating a latitudinal genetic cline

Genetic variation across life stage: examining the role of post-settlement selection

After confirming the presence of genetic structure across the range, we next examine the possible role of post-settlement selection in shaping this genetic structure. We found no significant genetic divergence between pre- and post-winter spat samples (Table 2). When comparing spat and adults collected from the same sampling location, we found no significant genetic divergence between life stages in CHE, whereas life stages were significantly divergent in Passamaquoddy Bay, NB (PSB; FST = 0.009, p < 0.001; see Table S3). The limited differences between temporal samples were further confirmed using a neighbor-joining tree, as samples from the same location generally grouped close together, including both life stages from PSB (see Figure S1).

Table 2 Pairwise genetic divergence (FST) and significance (p value) between pre- and post-winter sea scallop (Placopecten magellanicus) spat collected from the same sampling location

Environmental factors driving population structure

RDA identified nine environmental variables that explained genetic variation (Fig. 4a), and six of these variables were significant (p values < 0.05). The RDA was globally significant (F= 3.098; p = 0.001) with the first and second RDA axes explaining 16.5% and 6.7% of the genetic variation, respectively. Cold temperatures, including winter bottom temperature (BT; F = 2.24; p = 0.035), minimum sea surface temperature (SST; F = 2.38; p = 0.021), and winter SST (F = 3.30; p = 0.002), were significant and separated populations along the first RDA axis. Salinity variables were also significant, including minimum sea surface salinity (SSS; F = 2.52; p = 0.020), spring SSS (F = 2.38; p = 0.018), and fall bottom salinity (BS; F = 2.50; p = 0.016), and these variables generally separated inshore sites in NS and sites in the Gulf of St. Lawrence from other regions (Fig. 4a). When geography was accounted for in the pRDA, the same six environmental variables were significant (p values < 0.05). Environment and geography explained 58.2% and 8.9% of the genetic variation, respectively.

Fig. 4
figure4

Results of genetic–environment associations for sea scallop (Placopecten magellanicus) using population allele frequencies including (a) redundancy analysis and (b, c) random forest regression. a Redundancy analysis shows all selected environmental variables with arrow colors corresponding to salinity (green) and temperature (blue) measures, and site data point are colored based on their location being north (blue) or south (red) of the previously described genetic breakpoint. For random forest regressions, (b) the mean decrease in accuracy (MDA) for each environmental variable and (c) site-specific mean squared error (MSE) were averaged across all runs and loci

Consistent with the RDA, random forest (RF) regressions generally identified colder temperatures as important, where minimum BT, winter BT, minimum SST, as well as spring SST had the highest values for mean decrease in accuracy (Fig. 4b). The majority of other SST measures also showed high mean decrease in accuracy, whereas salinity measures were generally less important, particularly SSS measures (Fig. 4b). Sites near the extremes of the geographic range showed higher mean square errors (Fig. 4c) corresponding to the clinal structure of sea scallops.

Given the importance of cold temperatures in both analyses, the results of Bayesian clustering were overlaid on a map with mean winter BT (Fig. 1b) as it was identified in both analyses and is ecologically relevant to this benthic species. The relationship between admixture coefficient (Q value) and winter BT was also modeled using a generalized linear model (Fig. 5; p < 0.01).

Fig. 5
figure5

Relationship between genetic structure (admixture coefficient; Q value with standard error) and average winter bottom temperature across sea scallop (Placopecten magellanicus) populations modeled as a generalized linear model. Site names are indicated near each data point and colored based on latitude. Vertical dashed line represents a possible thermal threshold (1.5 °C) delineating the majority of the sites between the southern and northern genetic clusters (see Discussion)

Discussion

Understanding connectivity in the ocean is essential to fishery management and conservation. Recent advances in population genetics and genomics increasingly reveal unexpected fine-scale heterogeneity in marine species despite high dispersal potential (Reiss et al. 2009; Gagnaire et al. 2015). Here, targeted genetic analyses and expanded geographic coverage confirmed previous observations of northern and southern groups of sea scallop within the northwest Atlantic Ocean (Van Wyngaarden et al. 2017; 2018) but also revealed novel fine-scale structure between inshore and offshore sea scallop populations. Observations of climate-associated spatial structuring observed between inshore and offshore locations extend previous reports of large-scale latitudinal structure, and add further support to the hypothesis that gene flow in this species is associated with marine climate. Our work adds to the growing evidence of fine-scale genetic structure in marine species and provides valuable information for management of the economically significant sea scallop.

Fine-scale genetic structure

In our study we identified novel fine-scale genetic structure between inshore and offshore scallop populations. Previous work has clearly identified discrete northern and southern clusters of sea scallop populations with a boundary off eastern NS (Van Wyngaarden et al. 2017). Interestingly, we observed samples in both the northern and southern regions that unexpectedly clustered with the non-local group. In the northern region, scallops from the offshore site St. Pierre Bank unexpectedly grouped with the southern genetic cluster, and contrasted nearby (≥181 km distance) inshore sites in NL that grouped with the northern genetic cluster. This was unexpected, given St. Pierre Bank’s northern location (~46.5°N) and because a previous microsatellite-based study did not identify differences between St. Pierre Bank and another NL site (Kenchington et al. 2006). Furthermore, ocean currents in this region would be expected to facilitate larval connectivity between St. Pierre Bank and nearby northern locations (Naidu and Anderson 1984; Townsend et al. 2004). It has been suggested that STB is a highly variable stock with sporadic recruitment (Naidu and Anderson 1984) potentially explaining differences between our study and previous work in which STB scallops were sampled multiple decades earlier (Kenchington et al. 2006). Therefore, we acknowledge the possibility that the southern signature detected at STB may reflect stochastic effects that can only be resolved with additional temporal genetic sampling; nonetheless, our results for STB are consistent with the hypothesis of temperature-mediated genetic structure (discussed below).

In the southern region, again unexpected fine-scale structure was observed between inshore and offshore locations, with the clustering of inshore NS sites, specifically Chester (~44.5°N) and Chedabucto (~45.5°N), with the northern genetic group. This again contrasted nearby (≥128 km distance) offshore sites at similar latitudes (~44–44.5°N) on the eastern Scotian Shelf, which clustered with the southern group. In both instances (i.e., north and south), observations of unexpected differentiation between these offshore and inshore sites occur over distances that are within or below the predicted or estimated effective dispersal distances of sea scallop (Van Wyngaarden et al. 2017), suggesting that mechanisms other than passive larval dispersal alone are likely contributing to this previously uncharacterized fine-scale genetic structure.

No previous genetic studies have characterized the differences between inshore and offshore sea scallops found here, although complex patterns of genetic differentiation within offshore or within inshore sea scallop populations have been previously documented (Kenchington et al. 2006; Owen and Rawson, 2013). For example, significant genetic differentiation was found between closely spaced (~90 km) inshore locations in the Gulf of Maine, potentially resulting from sweepstakes recruitment or oceanographic features (Owen and Rawson 2013). In addition, fine-scale genetic differences were found by Kenchington et al. 2006 between scallops at adjacent (300 km) offshore sites on Georges Bank, although no specific mechanism could explain this isolation. Unlike Kenchington et al. 2006, we found no differences between adjacent (240 km) offshore sites on Georges Bank (GEO and NGB sites in our study). Our findings are consistent with other studies that did not find limited connectivity (Tremblay et al. 1994; Gilbert et al. 2010; Davies et al. 2014) or genetic structure (Van Wyngaarden et al. 2017) on Georges Bank. We acknowledge that some genetic structure present within local regions (i.e., within the southern and within the northern genetic clusters) identified using neutral markers (Kenchington et al. 2006; Owen and Rawson 2013) may not be as easily identified using our diagnostic SNPs, which were chosen to discriminate between southern and northern clusters. Even so, we still detected strong genetic differentiation (i.e., north vs. south clustering and high FST) at small geographic scales within regions. Altogether, our results support the hypothesis that significant population structure exists in sea scallop in the northwest Atlantic Ocean and that mechanisms other than larval dispersal alone likely underpin this structure.

Factors contributing to genetic structure

Admittedly, fine-scale structure can be driven by natural selection, where adaptation to the local environment can facilitate genetic divergence over small spatial scales (Clarke et al. 2010; Bradbury et al. 2013; Milano et al. 2014; Silva et al. 2014). Previous studies in sea scallops have identified biological differences across depths including variation in growth rate and reproductive output likely related to variation in food availability and temperature (Barber et al. 1988; MacDonald and Thompson 1988). Indeed, cold temperatures have been suggested as a potential driver of genetic variation (Van Wyngaarden et al. 2018). In our study, both redundancy analyses and random forest regressions support the role of cold temperatures in mediating genetic structure in sea scallop (see Fig. 1b). Interestingly, salinity variables were also identified in the redundancy analyses, particularly for differentiating sites in the Gulf of St. Lawrence and inshore NS from sites in other geographic regions; however, variation in salinity across the latitudinal range does not differentiate southern and northern genetic structure.

Our results strengthen the conclusions drawn by Van Wyngaarden et al. 2018, suggesting that the coldest temperatures (winter and annual minima) experienced by scallops drive differences between northern and southern clusters, consistent with temperature-mediated genetic structure of several other species in the North Atlantic (Bradbury et al. 2010; Jorde et al. 2015; Benestan et al. 2016; Jeffery et al. 2018). Differences in thermal tolerance have been detected between scallops collected from locations within the southern (Passamaquoddy Bay; PSB) and northern (Gulf of St. Lawrence) genetic clusters of our study (Dickie 1958). Here we suggest that cold temperatures may also facilitate fine-scale genetic differences between the inshore and offshore scallops, where an important thermal threshold may exist near 1.5 °C based on average winter BT. The majority of stocks assigned to the southern genetic cluster were found at locations where winter BT exceeded 1.5 °C (exceptions being two inner Bay of Fundy sites: BFU and BFS), and all stocks assigned to the northern genetic cluster were found at locations with average winter BTs below 1.5 °C (see Fig. 5).

Interestingly, our attempts to explore the temporal stability of these patterns across the early-life history suggest no significant changes in the proportion of north and south juvenile scallop (spat) collected pre- and post winter. This may be somewhat inconsistent with a hypothesis of high dispersal and strong selection driving the patterns observed. However, we acknowledge that the site locations, sampling year, and genetic markers used in our study may complicate the interpretation of these results. For example, for a change in the proportion of groups present in a sample to be detected, a site would need recruits from both northern and southern clusters, and this may be unlikely for many of these sampling sites. In addition, temporal genetic differences (i.e., between adults and juveniles collected in different years) were found at one site in our study (PSB), and thus, given the complex population dynamics of scallops, further examination, and additional temporal comparisons across multiple spatial scales may be warranted. Future experimental studies are needed to determine whether cold tolerance drives adaptive differences between scallop populations.

Management implications

Sea scallops represent an economically significant fishery in Canada and the US (NOAA, 2016; DFO, 2016a), and many of our sampling sites represent different management areas in North America (DFO, 2014a, 2014b, 2015; NOAA, 2017). Although we show fine-scale genetic differences between sites at higher latitudes, the current management areas correspond well to the genetic structure in our study, and inshore and offshore sites are managed separately (DFO, 2014a, 2015, 2016b). However, given the observed importance of temperature to the genetic structure of sea scallop and other marine organisms in the northwest Atlantic Ocean, the potential influence of climate change on species distribution may vary intraspecifically among southern and northern groups as predicted for several invertebrate and fish species (Le Bris et al. 2018; Stanley et al. 2018). Specifically, in sea scallops, forecasting models predict a greater increase in the spatial extent of suitable habitat for the northern genetic cluster relative to the southern group (Stanley et al. 2018). Therefore, clear resolution of the current spatial genetic structure and environmental associations can facilitate improved understanding and predictions of population responses to climate change.

Summary

In our study, we identified significant global and local genetic structure in sea scallops over the largest geographic range to date. The delineation between northern and southern genetic clusters of sea scallop was consistent with previous observations of sea scallop (Kenchington et al. 2006; Van Wyngaarden et al. 2017), as well as northern shrimp (Pandalus borealis; Jorde et al. 2015), green crab (Carcinus maenas; Jeffery et al. 2017), Atlantic cod (Bradbury et al. 2010), and American lobster (Homarus americanus; Benestan et al. 2016) in the northwest Atlantic Ocean (see Stanley et al. 2018). However, our results show that the boundary between northern and southern groups is not a discrete latitudinal break (Van Wyngaarden et al. 2017), as we show that variation in cold temperature around this boundary mediates population structure and aligns with fine-scale differences between inshore and offshore scallops. In conclusion, our results provide valuable fine-scale information for the management of sea scallop populations, highlighting the potential value of using environmental data to predict genetic structure, which can help create management actions that match biological units.

Data Archiving

Genotype data for 96 SNPs and environmental data are available from the Dryad Digital Repository https://doi.org/10.5061/dryad.59g2911

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Acknowledgements

We thank the people and organizations who contributed scallop samples, including NOAA (Devora Hart, Jakub Kircun), Maine Department of Marine Resources (Kevin Kelly), Fisheries and Oceans Canada (Amy Glass, Alan Reeves, Kyle Matheson, Leslie-Anne Davidson, Shawn Robinson, Leslie Nasmith, Andrew Cooper, Elizabeth Coughlan, Phil Sargent and Carole Turbide) and Sam Truesdale. We also thank the staff of the Aquatic Biotechnology Lab at the Bedford Institute of Oceanography for assistance with DNA extractions, RAD library preparation and SNP genotyping, and Praveen Nadukkalam Ravindran for bioinformatic support. This work was supported by a National Sciences and Engineering Research Council of Canada (NSERC) visiting fellowship awarded to S.J.L.

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Lehnert, S.J., DiBacco, C., Van Wyngaarden, M. et al. Fine-scale temperature-associated genetic structure between inshore and offshore populations of sea scallop (Placopecten magellanicus). Heredity 122, 69–80 (2019). https://doi.org/10.1038/s41437-018-0087-9

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