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Genetic control and evolutionary potential of a constitutive resistance mechanism against the spruce budworm (Choristoneura fumiferana) in white spruce (Picea glauca)

Abstract

Insect herbivory may drive evolution by selecting for trees with heritable resistance against defoliation. The spruce budworm (Choristoneura fumiferana, SBW) is a highly damaging forest insect pest that can affect population structure of white spruce (Picea glauca) in North America. Resistance against SBW was recently described in white spruce and was linked to three constitutive resistance biomarkers: the phenolic compounds piceol and pungenol, and expression of a beta-glucosidase encoding gene (Pgβglu-1). We investigated the phenotypic variability and heritability of these resistance biomarkers and of picein, the precursor of piceol, in the foliage of 874 trees belonging to 33 full-sib families and 71 clonal lines under evaluation in seven field locations in Eastern Canada. We aimed to (i) determine their genetic control, (ii) estimate the genetic and phenotypic correlations among defense biomarkers, and (iii) determine whether their constitutive levels are associated with detrimental trade-offs on growth. Quantitative genetics analyses indicated that all four traits are moderately to highly heritable. The full-sib and clonal analyses showed that additive and non-additive genetic effects play major and minor roles, respectively. Positive genetic and phenotypic correlations between resistance biomarkers and primary growth indicated that there is no trade-off between total height and height increment and resistance traits, contradicting the GDBH (Growth Differentiation Balance Hypothesis). Our findings about the predominant additive genetic basis of the resistance biomarkers show that adaptive evolution of white spruce natural populations to resist to SBW is possible and that potentially important gains could also be expected from artificial selection.

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Acknowledgements

We thank the Ministère des Forêts, de la Faune et des Parcs du Québec and JD Irving Ltd for access to field tests, permission to carry out sampling, and use of growth data. We thank Isabelle Giguère and Sébastien Caron (Université Laval) for technical assistance and laboratory support. We thank Gabriel Piette-Lauzière and Gaby Germanos (Université Laval) for assistance during field work and, Karen Guay and Pierre Audet (Université Laval) for assistance in chemical analysis.

Funding

Financial support was received from the Fonds de Recherche du Québec—Nature et Technologie (JM, ÉB), from NSERC of Canada for a strategic grant (JM) and INIFAP for supplementary funding for Ph.D. studies (CM-E).

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Conflict of interest

The authors declare that they have no conflict of interest.

Correspondence to Claudia Méndez-Espinoza.

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Provenances of Quebec progeny trials

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