Albinism is a clinically and genetically heterogeneous condition. Despite analysis of the 20 known genes, ~30% patients remain unsolved. We aimed to identify new genes involved in albinism.
We sequenced a panel of genes with known or predicted involvement in melanogenesis in 230 unsolved albinism patients.
We identified variants in the Dopachrome tautomerase (DCT) gene in two patients. One was compound heterozygous for a 14-bp deletion in exon 9 and c.118T>A p.(Cys40Ser). The second was homozygous for c.183C>G p.(Cys61Trp). Both patients had mild hair and skin hypopigmentation, and classical ocular features. CRISPR-Cas9 was used in C57BL/6J mice to create mutations identical to the missense variants carried by the patients, along with one loss-of-function indel. When bred to homozygosity the three mutations revealed hypopigmentation of the coat, milder for Cys40Ser compared with Cys61Trp or the frameshift mutation. Histological analysis identified significant hypopigmentation of the retinal pigmented epithelium (RPE) indicating that defective RPE melanogenesis could be associated with eye and vision defects. DCT loss of function in zebrafish embryos elicited hypopigmentation both in melanophores and RPE cells.
DCT is the gene for a new type of oculocutaneous albinism that we propose to name OCA8.
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The authors are grateful to the French Albinism Association Genespoir for financial support and for timeless action in favor of patients with albinism. I.G., L.M., M.K., and I.J.J. were funded by the MRC University Unit award to the MRC Human Genetics Unit, grant number MC_UU_00007/4. The authors also thank the patients for participating in this study and Angus Reid for assisting with colorimetric measurements.
The authors declare no conflicts of interest.
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
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Pennamen, P., Tingaud-Sequeira, A., Gazova, I. et al. Dopachrome tautomerase variants in patients with oculocutaneous albinism. Genet Med (2020). https://doi.org/10.1038/s41436-020-00997-8
Pigment Cell & Melanoma Research (2020)