Alopecia areata is an autoimmune disease in which activation of autoreactive T cells and inflammatory immune signals target the hair follicles autoantigens. Although cytokines are involved in regulating autoimmune inflammation, the specific involvement of these molecules in the pathogenesis of alopecia areata has been remained unsettled. Here, a possible influence of IL12B, IL17A, and IL23R variations on susceptibility to alopecia areata in Iranian patients was investigated. Genotyping of IL12B (rs3212227), IL17A (rs2275913), and IL23R (rs10889677) variants were performed by extracting genomic DNA from patients and controls. Gene expression was analyzed by real-time RT-PCR. The frequency of IL12B and IL23R gene polymorphisms is significantly higher in the patients than controls, while no significant difference was found for IL17A. Stratification of the patients with respect to age at disease onset indicated that CC genotype of IL12B (rs3212227) and AA genotype of IL23R (rs10889677) gene polymorphisms are significantly associated with late-onset alopecia areata disease. In contrast to IL17A and IL23R, IL12B gene expression levels elevated in patients to that of controls, but genotypes had no effect on levels of gene expression. Overall, our data confirmed that the IL12B and IL23R polymorphisms are associated with the risk to develop alopecia areata in our population.
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We would like to thank the Medical and Nursing staff of the Skin Research Center, Shohada Tajrish and Loghman Hakim hospitals at Tehran, Iran for helpful collaboration.
Conflict of interest
The authors declare that they have no conflict of interest.
The study protocol was considered and approved by the local ethics committee (Human Research Ethics Committee of Skin Research Center, Shahid Beheshti University of Medical Sciences). The investigation conforms to the principles outlined in the 1964 Declaration of Helsinki, revised in 2000.
Informed patient consent was obtained from all individuals.
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Tabatabaei-Panah, PS., Moravvej, H., Delpasand, S. et al. IL12B and IL23R polymorphisms are associated with alopecia areata. Genes Immun 21, 203–210 (2020). https://doi.org/10.1038/s41435-020-0100-1
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