Fig. 2: eGFP mRNA expression in the CNS tissues in C57BL/6J mice, following neonatal injection of AAV-PHP.eB-CBA-eGFP. | Gene Therapy

Fig. 2: eGFP mRNA expression in the CNS tissues in C57BL/6J mice, following neonatal injection of AAV-PHP.eB-CBA-eGFP.

From: Use of CRISPR/Cas9-mediated disruption of CNS cell type genes to profile transduction of AAV by neonatal intracerebroventricular delivery in mice

Fig. 2

Representative RNAscope images showed eGFP mRNA expression, shown in violet, and cell nuclei, shown in blue, in various CNS regions in C57BL/6J mice injected with AAV-PHP.eB (ak) and PBS-injected control mice (lv): a sagittal section of the whole brain (a and l), olfactory bulb (b and m), cortex (c and n), hippocampus (d and o), corpus callosum (e and p), substantia nigra pars compacta (f and q), a full transverse section of the spinal cord (g and r), thalamus (h and s), cerebellum (i and t), gray matter area of the ventral spinal cord (j and u), and white matter area of the spinal cord (k and v). All scale bars are 50 µm.

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