Skip to main content

Thank you for visiting You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Comment
  • Published:

Gene conversion following CRISPR/Cas9 DNA cleavage: an overlooked effect

This is a preview of subscription content, access via your institution

Relevant articles

Open Access articles citing this article.

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout


  1. Jinek M, Chylinski K, Fonfara I, Hauer M, Doudna JA, Charpentier E. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science. 2012;337:816–21.

    Article  CAS  Google Scholar 

  2. Stark JM, Pierce AJ, Oh J, Pastink A, Jasin M. Genetic steps of mammalian homologous repair with distinct mutagenic consequences. Mol Cell Biol. 2004;24:9305–16.

    Article  CAS  Google Scholar 

  3. Ma H, Marti-Gutierrez N, Park SW, Wu J, Lee Y, K. Suzuki A, et al. Correction of a pathogenic gene mutation in human embryos. Nature. 2017;548:413–9.

    Article  CAS  Google Scholar 

  4. Adikusuma F, Piltz S, Corbett MA, Turvey M, McColl SR, Helbig KJ, R M, et al. Large deletions induced by Cas9 cleavage. Nature. 2018;560:E8–9.

    Article  CAS  Google Scholar 

  5. Egli D, Zuccaro MV, Kosicki M, Church GM, Bradley A, Jasin M. Inter-homologue repair in fertilized human eggs? Nature. 2018;560:E5–7.

    Article  CAS  Google Scholar 

  6. Javidi-Parsijani P, Lyu P, Makani V, Sarhan WM, Yoo KW, El-Korashi L, et al. CRISPR/Cas9 increases mitotic gene conversion in human cells. Gene Ther. 2020.

  7. Papadakis MN, Patrinos GP. Contribution of gene conversion in the evolution of the human beta-like globin gene family. Hum Genet. 1999;104:117–25.

    Article  CAS  Google Scholar 

  8. Katic I, Grosshans H. Targeted heritable mutation and gene conversion by Cas9-CRISPR in Caenorhabditis elegans. Genetics. 2013;195:1173–6.

    Article  CAS  Google Scholar 

  9. Wilde JJ, Aida T, Wienish M, Zhang Q, Qi P, Feng G. Efficient zygotic genome editing via RAD51-enahnced interhomolog repair. BioRxiv 2018;263699.

Download references


GB receives funding from the National collaborative Research Infrastructure (NCRIS) via the Australian Phenomics Network, National Health and Medical Research Council of Australia (APP1143008), the Australian Research Council (DP180101494), and the National Natural Science Foundation of China (81772214).

Author information

Authors and Affiliations


Corresponding author

Correspondence to Gaetan Burgio.

Ethics declarations

Conflict of interest

The author declares that he has no conflict of interest.

Additional information

Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Burgio, G. Gene conversion following CRISPR/Cas9 DNA cleavage: an overlooked effect. Gene Ther 27, 245–246 (2020).

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI:

This article is cited by


Quick links