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References
Jinek M, Chylinski K, Fonfara I, Hauer M, Doudna JA, Charpentier E. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science. 2012;337:816–21.
Stark JM, Pierce AJ, Oh J, Pastink A, Jasin M. Genetic steps of mammalian homologous repair with distinct mutagenic consequences. Mol Cell Biol. 2004;24:9305–16.
Ma H, Marti-Gutierrez N, Park SW, Wu J, Lee Y, K. Suzuki A, et al. Correction of a pathogenic gene mutation in human embryos. Nature. 2017;548:413–9.
Adikusuma F, Piltz S, Corbett MA, Turvey M, McColl SR, Helbig KJ, R M, et al. Large deletions induced by Cas9 cleavage. Nature. 2018;560:E8–9.
Egli D, Zuccaro MV, Kosicki M, Church GM, Bradley A, Jasin M. Inter-homologue repair in fertilized human eggs? Nature. 2018;560:E5–7.
Javidi-Parsijani P, Lyu P, Makani V, Sarhan WM, Yoo KW, El-Korashi L, et al. CRISPR/Cas9 increases mitotic gene conversion in human cells. Gene Ther. 2020. https://doi.org/10.1038/s41434-020-0126-z.
Papadakis MN, Patrinos GP. Contribution of gene conversion in the evolution of the human beta-like globin gene family. Hum Genet. 1999;104:117–25.
Katic I, Grosshans H. Targeted heritable mutation and gene conversion by Cas9-CRISPR in Caenorhabditis elegans. Genetics. 2013;195:1173–6.
Wilde JJ, Aida T, Wienish M, Zhang Q, Qi P, Feng G. Efficient zygotic genome editing via RAD51-enahnced interhomolog repair. BioRxiv 2018;263699. https://doi.org/10.1101/263699.
Funding
GB receives funding from the National collaborative Research Infrastructure (NCRIS) via the Australian Phenomics Network, National Health and Medical Research Council of Australia (APP1143008), the Australian Research Council (DP180101494), and the National Natural Science Foundation of China (81772214).
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Burgio, G. Gene conversion following CRISPR/Cas9 DNA cleavage: an overlooked effect. Gene Ther 27, 245–246 (2020). https://doi.org/10.1038/s41434-020-0154-8
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DOI: https://doi.org/10.1038/s41434-020-0154-8