miR-19a/19b improves the therapeutic potential of mesenchymal stem cells in a mouse model of myocardial infarction

Abstract

Myocardial infarction (MI) is the cardiac emergency that may leads to myocardial necrosis. Mesenchymal stem cells (MSCs) could be used to induce myocardial differentiation. However, the efficiency remains low. The aim of this study is to explore whether miR-19a/19b could enhance the therapeutic potential of mesenchymal stem cells in MI. Myocardial infarction mouse model was established using coronary artery ligation. Cardiac functional recovery was detected by Masson’s trichrome staining. Under hypoxic condition, miR-19a/19b expression levels decreased in bone marrow-derived MSCs (BM-MSCs). MiR-19a/19b suppressed the proliferation of MSCs under hypoxic condition. After cell engraftment, miR-19a/19b promoted survival of MSCs. Mechanically, miR-19a/19b inhibited inflammatory cells infiltration into myocardium cells. Moreover, MSCs-miR-19a/19b improves cardiac functional recovery in diabetic MI mice models. All the results indicated that miR-19a/19b improves the therapeutic potential of mesenchymal stem cells in a mouse model of myocardial infarction.

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Fig. 1: Characterization of BM-MSCs and miR-19a/19b expression under hypoxic condition.
Fig. 2: miR-19a/19b facilitates survival of BM-MSCs under hypoxic condition.
Fig. 3: miR-19a/19b facilitates survival of BM-MSCs after cell engraftment.
Fig. 4: Transplantation of MSCs-miR-19a/19b suppresses infiltration of inflammatory cells and expression of pro-inflammatory cytokines in the myocardium.
Fig. 5: Transplantation of MSCs-miR-19a/19b improves cardiac functional recovery in mice model of diabetic MI.

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Correspondence to Chengbo Chen.

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Chen, C., Chen, T., Li, Y. et al. miR-19a/19b improves the therapeutic potential of mesenchymal stem cells in a mouse model of myocardial infarction. Gene Ther (2020). https://doi.org/10.1038/s41434-020-0122-3

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