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Structural integrity of retinal pigment epithelial cells in eyes with age-related scattered hypofluorescent spots on late phase indocyanine green angiography (ASHS-LIA)

Eye volume 37, pages 377–378 (2023)Cite this article

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Fig. 1: Frequency of ASHS-LIA in our cohort.
Fig. 2: The alternating bright and dark pattern (ASHS-LIA), not evident on optical coherence tomography (OCT), was visible using late phase ICGA in a healthy eye (63-year-old) in both conventional and adaptive optics imaging.

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All data generated or analyzed during this study are included in this article.

References

  1. Chen L, Yang P, Curcio CA. Visualizing lipid behind the retina in aging and age-related macular degeneration, via indocyanine green angiography (ASHS-LIA). Eye. 2022;36:1735–46.

  2. Chen L, Zhang X, Li M, Gan Y, Wen F. Drusen and age-related scattered hypofluorescent spots on late-phase indocyanine green angiography, a candidate correlate of lipid accumulation. Invest Ophthalmol Vis Sci. 2018;59:5237–45.

    Article  CAS  Google Scholar 

  3. Shiraki K, Moriwaki M, Kohno T, Yanagihara N, Miki T. Age-related scattered hypofluorescent spots on late-phase indocyanine green angiograms. Int Ophthalmol. 1999;23:105–9.

    Article  CAS  Google Scholar 

  4. Tam J, Liu J, Dubra A, Fariss R. In vivo imaging of the human retinal pigment epithelial mosaic using adaptive optics enhanced indocyanine green ophthalmoscopy. Invest Ophthalmol Vis Sci. 2016;57:4376–84.

    Article  CAS  Google Scholar 

  5. Jung H, Liu J, Liu T, George A, Smelkinson MG, Cohen S, et al. Longitudinal adaptive optics fluorescence microscopy reveals cellular mosaicism in patients. JCI Insight. 2019;4:e124904.

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Acknowledgements

The authors would like to thank Alfredo Dubra for assistance with adaptive optics instrumentation and software, and Mike Arango, Denise Cunningham, HaeWon Jung, Jianfei Liu, Rongwen Lu, Christina Appleman, Jenny Suy, Sharon Yin, John Rowan, Patrick Lopez, Guy Foster, Gloria Babilonia-Ayukawa, Daniel Claus, and Angel Garced for assistance with study procedures. This work was supported by the Intramural Research Program of the National Institutes of Health, National Eye Institute.

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  1. National Eye Institute, National Institutes of Health, Bethesda, MD, USA

    Joanne Li, Nancy Aguilera, Tao Liu, Andrew J. Bower, John P. Giannini, Catherine Cukras, Tiarnan D. L. Keenan, Emily Chew, Brian P. Brooks, Wadih M. Zein, Laryssa A. Huryn, Robert B. Hufnagel & Johnny Tam

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  1. Joanne Li
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Contributions

JL and JT contributed to the design and writing of the letter. JL, NA, and JT contributed to data analysis and discussion. JL, NA, TL, AJB, JPG, and JT contributed to data collection. CC, TK, EC, BPB, WMZ, LAH, and RBH contributed to patient referral and clinical examination. All authors reviewed the final draft.

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Correspondence to Johnny Tam.

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Li, J., Aguilera, N., Liu, T. et al. Structural integrity of retinal pigment epithelial cells in eyes with age-related scattered hypofluorescent spots on late phase indocyanine green angiography (ASHS-LIA). Eye 37, 377–378 (2023). https://doi.org/10.1038/s41433-022-02232-x

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