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Changes in volume of various retinal layers over time in early and intermediate age-related macular degeneration

Eyevolume 33pages428434 (2019) | Download Citation



To evaluate longitudinally volume changes in inner and outer retinal layers in early and intermediate age-related macular degeneration (AMD) compared to healthy control eyes using optical coherence tomography (OCT).


71 eyes with AMD and 31 control eyes were imaged at two time points: baseline and after 2 years. Automated OCT layer segmentation was performed using OrionTM. This software is able to measure volumes of retinal layers with distinct boundaries including Retinal Nerve Fibre Layer (RNFL), Ganglion Cell-Inner Plexiform Layer (GCIPL), Inner Nuclear Layer (INL), Outer Plexiform Layer (OPL), Outer Nuclear Layer (ONL), Photoreceptors (PR) and Retinal Pigment Epithelium–Bruch’s Membrane complex (RPE-BM). The mean retinal layer volumes and volume changes at 2 years were compared between groups.


Mean GCIPL and INL volumes were lower, while PR and RPE-BM volumes were higher in AMD eyes than controls at baseline (all P < 0.05) and year 2 (all P < 0.05). In AMD eyes, RNFL and ONL volumes decreased by 0.0232 (P = 0.033) and 0.0851 (P = 0.001), respectively. In contrast, OPL and RPE-BM volumes increased in AMD eyes by 0.0391 (P = 0.000) and 0.0209 (P = 0.000) respectively. Moreover, there were significant differences in longitudinal volume change of OPL (P = 0.02), ONL (P = 0.008) and RPE-BM (P = 0.02) between AMD eyes and controls.


There were abnormal retinal layer volumes and volume changes in eyes with early and intermediate AMD.

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The research was supported by the National Institute for Health Research (NIHR) Biomedical Research Centre based at Moorfields Eye Hospital NHS Foundation Trust and UCL Institute of Ophthalmology. The views expressed are those of the author(s) and not necessarily those of the NHS, the NIHR or the Department of Health.

Author information


  1. NIHR Moorfields Biomedical Research Centre, Moorfields Eye Hospital NHS Foundation Trust, London, UK

    • Ali Lamin
    • , Adam M. Dubis
    •  & Sobha Sivaprasad
  2. UCL Institute of Ophthalmology, London, UK

    • Ali Lamin
    • , Adam M. Dubis
    •  & Sobha Sivaprasad
  3. Voxeleron LLC, Pleasanton, CA, USA

    • Jonathan D. Oakley
    •  & Daniel B. Russakoff


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The authors declare that they have no conflict of interest.

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Correspondence to Sobha Sivaprasad.

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