Microsatellite instability screening in colorectal adenomas to detect Lynch syndrome patients? A systematic review and meta-analysis

Abstract

The colorectal cancer spectrum has changed due to population screening programs, with a shift toward adenomas and early cancers. Whether it would be a feasible option to test these adenomas for detection of Lynch syndrome (LS) patients is unclear. Through meta-analysis and systematic review, risk factors for DNA mismatch repair deficiency (dMMR) and microsatellite instability (MSI) in adenomas were identified in LS and unselected patient cohorts. Data were extracted for patient age and MMR variant together with adenoma type, grade, size, and location. A total of 41 studies were included, and contained more than 519 LS patients and 1698 unselected patients with 1142 and 2213 adenomas respectively. dMMR/MSI was present in 69.5% of conventional adenomas in LS patients, compared with 2.8% in unselected patients. In the LS cohort, dMMR/MSI was more frequently present in patients older than 60 years (82% versus 54%). dMMR/MSI was also more common in villous adenomas (84%), adenomas over 1 cm (81%), and adenomas with high grade dysplasia (88%). No significant differences were observed for dMMR/MSI in relation to MMR variants and location of adenomas. In the context of screening, we conclude that detection of dMMR/MSI in conventional adenomas of unselected patients is uncommon and might be considered as indication for LS testing. Within the LS cohort, 69.5% of LS patients could have been detected through dMMR/MSI screening of their conventional adenomas.

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Data availability

Most data generated or analyzed during this study are included in this published article and its supplementary data. Additional information files are available from the corresponding author upon reasonable request.

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Funding

AB is supported by Netherlands Organization for Scientific Research (NWO) Veni grant 016.166.089. CEB and AB are supported by the Dutch cancer society (KWF kankerbestrijding) KUN 2015-7739. BED is supported by Netherlands Organization for Scientific Research (NWO) Vidi grant 864.14.004.

Author information

PDD, CEB, BED, AB, and IDN contributed to study design, data-acquisition, -analysis, and -interpretation, as well as drafting of the paper. AB and IDN supervised the project. All authors performed critical revision of the paper for important intellectual content.

Correspondence to Iris D. Nagtegaal.

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No ethical review board was involved in this meta-analysis, because public documents are subjects of the study. Data was collected through an unbiased search strategy with well-defined inclusion and exclusion criteria. Data was extracted, analyzed accurately and was tested for bias [65].

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Dabir, P.D., Bruggeling, C.E., van der Post, R.S. et al. Microsatellite instability screening in colorectal adenomas to detect Lynch syndrome patients? A systematic review and meta-analysis. Eur J Hum Genet 28, 277–286 (2020). https://doi.org/10.1038/s41431-019-0538-7

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