Fig. 3 | European Journal of Human Genetics

Fig. 3

From: A novel NAA10 variant with impaired acetyltransferase activity causes developmental delay, intellectual disability, and hypertrophic cardiomyopathy

Fig. 3

Activity measurements and turnover rate of NAA10-Ile72Thr-V5 versus NAA10-WT-V5. a Quantification of immunoprecipitated NAA10-WT-V5 and NAA10-Ile72Thr-V5 used in acetylation assays. Quantification of V5-bands were performed using the ChemiDocTM XRS+ system, and the ImageLab 5.1 software from Bio-RAD. b Catalytic activity of immunoprecipitated NAA10 variants. The catalytic activity was measured and correlated to the amount of NAA10 present in each sample as determined by densitometry analysis in a. c Western blot of cycloheximide chase experiments. Samples were Western blotted and analyzed with anti-V5, anti-NAA10, anti-NAA15, and anti-β-tubulin as a loading control. Anti-NAA10 recognize two main bands in the western blot, denoted * and **, bands marked with * corresponds to the expected size of V5-tagged NAA10 while ** corresponds to the expected size of endogenous untagged NAA10. d Band intensities of V5-tagged NAA10 variants were quantified as in a, and the value for each time point were correlated to the measured density of t0

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